Culture-independent real-time PCR assay for rapid enumeration of salmonella in seafood
Culture-independent real-time PCR assay for rapid enumeration of salmonella in seafood
Date
2010
Authors
Kumar, R.
Surendran, P.K.
Thampuran, N.
Lalitha, K.V.
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Fisheries Technologists (India)
Abstract
Salmonella is most important food-borne pathogen and commonly
associated with water and food-borne outbreaks. Approximately 1 .4 million
cases of salmonellosis were reported in the USA each year and similarly,
outbreaks were reported in the Asian and European countries (D'Aoust,
1989). The incidence of salmonellosis over a period (1988-1992) in USA
showed the 7.4% outbreaks were associated with the consumption of fish
or shellfish (Bean et al., 1997). Prevalence of Salmonella contamination
in seafood in imported and internal market of the United States was
reported in a ten year study from 1990 to 1998 by field laboratories of
USFDA and demonstrated that 11312 imported (7.1%) and 768 domestic
(1.3%) seafood samples were positive for Salmonella (Heinitz et al., 2000).
The contaminations in the coastal areas and the post harvest handling
in seafood have been found responsible for Salmonella contamination in
fish and seafood (Hatha and Lakshmanaperumalsamy, 1997; Kumar et
al., 2009). There is a report on influence of environmental factors and
human activity on the presence of Salmonella serovars in the marine
environments of Galicia region in Spain (Marti nez-Urtaza et al., 2004).
In most of the cases, Salmonella load in food and feed samples are
reported to be low in numbers (Seo et al., 2006; Boughton et al., 2007).
In such cases, sometime culture method may not be able to detect the
presence of Salmonella. Thus, it is important to quantify the presence of
contaminant in food items so as to establish the quantitative microbial risk
assessment measures.
Description
Keywords
PCR, salmonella, seafood, Indian mackerel, shrimp samples, chromagar method
Citation
Proceedings of the National Seminar on Conservation and Sustainability of Coastal Living Resources of India, 1-3 December 2009, Cochin, ed.by Meenakumari, B. et.al 606-615