Alterations in Body Fatty acid Composition in Microalga, Pavlova lutheri, grown in different standard Culture Media
Alterations in Body Fatty acid Composition in Microalga, Pavlova lutheri, grown in different standard Culture Media
Date
2021
Authors
Ganga, Rajani S.
Anandan
Korath, Alphi
Joseph, Aneykutty
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Fisheries Technologists (India)
Abstract
The present work monitored variations in fatty acid
profile and analysed the nutritional status of the
live feed Pavlova lutheri, a marine microalga which
was grown in three different culture media. Pure
cultures of microalga were collected from the
marine hatchery complex of the ICAR-Central
Marine Fisheries Research Institute, Kochi. Standard
algal culture conditions viz., medium, in sterilized
seawater of salinity 33 ppt, standard pH of 8.0,
culture room temperature of 25oC, light intensity of
1500 lux with a photoperiod of 12h.L:12h.D etc.
were maintained. Lipids were extracted and were
subjected to gas chromatography in an attempt to
ascertain their fatty acid profile. Total saturated fatty
acids ranged from 54.88% (Miquel’s medium),
76.54% (Chu#10 medium) to 75.8% (F/2). In the case
of monounsaturated, only a slight variation is seen
among the three. ie. minimum for Chu#10 (16.54%)
and maximum for F/2 (19.99%). For cultures in
Miquel’s, medium the value was 18.42%. Polyunsaturated
fatty acids (PUFAs) accounted for over
26.70% of the total fatty acids in Miquel’s medium
whereas it was only 6.87 and 4.21% in Chu#10 and
F/2 media respectively. Statistical analysis of data
brought to light that the composition of the medium
can influence the fatty acid pattern of microalgae
significantly (p<0.05). By analyzing the results it can
be confirmed that the nutritional value of Pavlova
lutheri can be enhanced by culturing in Miquel’s
medium.
Description
Keywords
Microalgae, Pavlova lutheri, F/2 medium, Miquel’s medium