Isolation and characterization of Listeria monocytogenes from tropical seafood of Kerala, India

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dc.contributor.authorDas, S.
dc.contributor.authorLalitha, K.V.
dc.contributor.authorThampuran, N.
dc.contributor.authorSurendran, P.K.
dc.date.accessioned2015-10-15T07:37:27Z
dc.date.available2015-10-15T07:37:27Z
dc.date.issued2013
dc.description.abstractListeria monocytogenes, which is an intracellular pathogen, causes various illnesses in human as well as in animals. The pathogenicity of this organism depends upon the presence of different virulence genes. A total of 324 tropical seafood and fishery environmental samples were screened for L. monocytogenes. The incidence of the human pathogenic species L. monocytogenes was 1.2 % of the samples. Listeria spp. was detected in 32.3, 27.1, and 5%of fresh, frozen, and dry fish samples, respectively. Listeria innocua was found to be the most prevalent species of Listeria in the tropical seafood and environmental samples of Kerala. Listeria monocytogenes and L. innocua isolates were confirmed by multiplex PCR. L. monocytogenes isolates from the four positive samples showed phosphatidylinositol-specific phospholipase C reaction on Chromocult® Listeria selective agar. Molecular characterization of L. monocytogenes isolates for virulence genes revealed the presence of β-hemolysin (hly), plcA, actA,metalloprotease (mpl), iap and prfA genes in all the isolates recovered from the positive samples.en_US
dc.identifier.citationAnnals of Microbiology 2013:63:1093–1098en_US
dc.identifier.urihttp://hdl.handle.net/123456789/2053
dc.language.isoenen_US
dc.publisherspringeren_US
dc.subjectListeria monocytogenesen_US
dc.subjectTropicalen_US
dc.subjectseafooden_US
dc.subjectVirulence geneen_US
dc.titleIsolation and characterization of Listeria monocytogenes from tropical seafood of Kerala, Indiaen_US
dc.typeArticleen_US
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