Characterization and Evaluation of Selected House-keeping Genes for Quantitative RT-PCR in Macrobrachium rosenbergii Morphotypes

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dc.contributor.authorPriyadarshi, Himanshu
dc.contributor.authorDas, Rekha
dc.contributor.authorKumar, A. Pavan
dc.contributor.authorP, Gireesh Babu
dc.contributor.authorJaved, Hasan
dc.contributor.authorKrishna, Gopal
dc.contributor.authorMarappan, Makesh
dc.contributor.authorChaudhari, Aparna
dc.date.accessioned2021-10-13T08:41:36Z
dc.date.available2021-10-13T08:41:36Z
dc.date.issued2015
dc.description.abstractMacrobrachium rosenbergii (De Man, 1879) is an important freshwater prawn cultured globally and is known to exhibit three male morphotypes with differential growth rates. Several gene expression studies using qRT-PCR have been undertaken in this species using any one of the popularly used internal control genes. Here we evaluate four house-keeping genes namely EF1a, GAPDH, b-actin and 18S rRNA for transcript stability across various M. rosenbergii tissues in male morphotypes for use as internal control genes in expression studies. Expression of these genes was measured in 5 tissues (androgenic gland, gill, eyestalk, nerve cord and testis) across morphotypes and Ct values were analyzed using four statistical methods (DCt, geNorm, BestKeeper and NormFinder) developed for this purpose. We report that EF1a is the most suited internal control for data normalization in androgenic gland, testis, nerve cord and gills while GAPDH is best for eyestalk tissue of M. rosenbergii irrespective of the morphotype. Wherever two reference genes are required b-actin can be included for all the tissues except in nerve cord, where GAPDH is more suitable second reference gene. M. rosenbergii EF1a and GAPDH partial mRNA sequences were also generated as part of this study and their phylogenetic relation with other decapods is also reported.en_US
dc.identifier.citationFishery Tech 52(3):177-183en_US
dc.identifier.urihttp://drs.cift.res.in/handle/123456789/5034
dc.language.isoenen_US
dc.publisherSociety of Fisheries Technologists (India)Cochinen_US
dc.subjectMacrobrachium rosenbergii, morphotypes, quantitative RT-PCR, house-keeping gene, normalization, reference genesen_US
dc.titleCharacterization and Evaluation of Selected House-keeping Genes for Quantitative RT-PCR in Macrobrachium rosenbergii Morphotypesen_US
dc.typeArticleen_US
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