Cultural Conditions of Arylsulfatase Activity in Escherichia coli
Cultural Conditions of Arylsulfatase Activity in Escherichia coli
Date
1986
Authors
DHEVENDARAN, K
CHANDRAMOHAN, D
NATARAJAN, R
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Fisheries Technologists (India) Cochin
Abstract
Arylsulfatase activity and growth were estimated in Escherichia coli, isolated from
marine sediment. Maximum activity was observed at pH 6.6 whereas the maximum
growth was at pH 5.6. 2 x 10-3 m M is the optimum substrate concentration for the
highest level of enzyme activity/synthesis as well as for its growth. In general higher
substrate concentration tended to inhibit enzyme activity and also the growth of the
bacterium. Maximum growth and highest enzyme activity occurred at 29°C and above
this temperature decreased both of them. Besides these, glucose, sodium sulfate, sodium
chloride, sodium dihydrogen phosphate, sodium acetate and ammonium chloride at
higher concentrations were inhibiting the enzyme activity and growth. Above 0.2% of
glucose, 3% of sodium chloride, 10 x 10-3 M concentrations of sodium sulfate, sodium
dihydrogen phosphate, sodium acetate and ammonium chloride inhibited the activity
and growth also. These observations indicate that, to generalise a compound as inhibitor
or activator it is difficult since this depends not only on its concentration but also
on the source of the enzyme when more than one type is encountered in nature.
Description
Keywords
Cultural Conditions, Arylsulfatase Activity ,Escherichia coli
Citation
Fishery Tech 23(1):158-163