Prevalence of molecular characterization of Vibrio cholerae 01, Non-01 and Non-0139 in tropical seafood, Cochin, India

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dc.contributor.authorKumar, R.
dc.contributor.authorLalitha, K.V.
dc.date.accessioned2015-08-24T05:08:05Z
dc.date.available2015-08-24T05:08:05Z
dc.date.issued2009
dc.description.abstractThe objective of this study was to determine the prevalence of 01, 0139, and non-01 and non-0139 Vibrio cholerae, which were associated with fresh and raw seafood samples harvested from Cochin, India waters during 2009-2011. Results from V. cholerae—specific biochemical, molecular, and serological assays identified five El Tor V. cholerae 01 Ogawa strains and 377 non-01, non-0139 V. cholerae strains from 265 seafood samples. V. cholerae 0139 strains were not isolated. Polymerase chain reaction assays confirmed the presence of V. cholerae 01 El Tor biotype in seafood. Antibiotic susceptibility analysis revealed that the V. cholerae 01 strains were pansusceptible to 20 test antibiotics, whereas 26%, 40%, 62%, and 84% of the non-01, non-0139 V. cholerae strains were resistant to cefpodoxirne, ticarcillin, augmentin, and colistin, respectively. Detection of virulence and regulatory genes in V. cholerae associated with seafood revealed the presence of virulence and regulatory genes (i.e., ctx, zot, ace, toxR genes) in V. cholerae 01 strains, nevertheless, presence of ace and toxR genes were detected in non-01, non-0139 in 9.8 and 91% strains, respectively. In conclusion, the presence of pathogenic V. cholerae in seafood harvested from local Cochin waters warrants the introduction of a postharvest seafood monitoring program, which will lead to a greater understanding of the distribution, abundance, and virulence of diverse pathogenic Vibrio populations that inhabit these different coastal regions so that a risk management program can be established.en_US
dc.identifier.citationFoodborne pathogens and disease 2013:10(3), 278-282en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1977
dc.language.isoenen_US
dc.publisherMary Ann Libert Incen_US
dc.subjectvibrio choleraeen_US
dc.subjectseafooden_US
dc.titlePrevalence of molecular characterization of Vibrio cholerae 01, Non-01 and Non-0139 in tropical seafood, Cochin, Indiaen_US
dc.typeArticleen_US
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