Virulence genes, serobiotypes and antibiotic resistance profile of Escherichia coli strains isolated from aquaculture and other sources

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dc.contributor.authorSurendraraj, A.
dc.contributor.authorThampuran, N.
dc.contributor.authorJoseph, T.C.
dc.date.accessioned2016-12-23T10:35:17Z
dc.date.available2016-12-23T10:35:17Z
dc.date.issued2010
dc.description.abstractIn order to determine the prevalence of pathogenic Escherichia coli, a total number of 155 E. coli isolates from aquaculture, clinical and veterinary sources were screened for seven pathogenic virulence markers and a house-keeping gene by a polymerase chain reaction. The targeted virulence genes included eaeA of enteropathogenic E. coli, elt and est of enterotoxigenic E. coli (ETEC), ipaH of enteroinvasive E. coli, pCVD432 of enteroaggregative E. coli, stx, hlyA and eaeA of shigatoxigenic E. coli (STEC) and Enterohaemorrhagic E. coli. All the isolates were positive for phoA, the house-keeping gene for E. coli. Among the 155 isolates, seven numbers (4.5%) harboured the virulencemarkers belonging to the pathogenic group ETEC and STEC. The virulent genes detected in these groups were elt, est, hlyAand stx.The sources of these virulence genes were ¢sh (hlyA), shrimp (elt), feeder canal water (hlyA and elt) of aquaculture origin and from diarrhoea a¡ected cow (hlyA, est and stx). The isolates with pathogenic traits belonged to the serogroups O6 or O29 and the remaining could not be typed. They showed resistance to two to four antibiotics out of the 12 antibiotics tested. Biotyping revealed that three isolates belonged to a single biotype (7333) and the remaining isolates were of diverse types. In conclusion, a molecular tool such as PCR proves as more e¡ective tool for detection of this pathogen than the conventional methods. Detection of these emerging pathogens in aquaculture samples warrants for strict adherence to hygienic handling at retail outlets and proper cooking by the consumer before consumptionen_US
dc.identifier.citationAquaculture Research 2010, 41: 1003-1014en_US
dc.identifier.urihttp://hdl.handle.net/123456789/2384
dc.language.isoenen_US
dc.publisherWileyen_US
dc.subjectE. colien_US
dc.subjectaquaculture farmsen_US
dc.subjectvirulence markeren_US
dc.subjectPCRen_US
dc.titleVirulence genes, serobiotypes and antibiotic resistance profile of Escherichia coli strains isolated from aquaculture and other sourcesen_US
dc.typeArticleen_US
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