Dietary L-Tryptophan potentiates non-specific immunity in Labeo rohita fingerlings reared under elevated temperature
Dietary L-Tryptophan potentiates non-specific immunity in Labeo rohita fingerlings reared under elevated temperature
Date
2018
Authors
Kumar, P.
Pal, A.K.
Sahu, N.P.
Jha, A.K.
Kumar, N.
Christina, L.
Priyadarshini, P.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
At present environmental scenario global climate change is a reality and its affect all living organism including
fish. The aquatic ecosystem is the most affected system as it is the biggest sink for global warming and elevated
temperature and obviously affects all the aquatic life forms. With this hypothesis an experiment was conducted
to evaluate the effect of elevated temperature on Labeo rohita fingerlings and potential of dietary L-tryptophan
(TRP) in mitigating the effects elevated temperature and enhancing the non-specific immunity. Seven hundred
and twenty fishes were randomly distributed in three different thermal groups each with three replicates. The
thermal groups were ambient temperature (26 °C), 34 and 38 °C. Then each thermal groups were fed with four
different formulated diets containing 0. 0.36%, 0.72% and 1.44% TRP. The effect of dietary TRP supplementation
was studied on stress responses, such as cortisol, blood glucose, histopathological changes in liver and
kidney and immuno-hematological changes such as red blood cell count (RBC), haemoglobin (Hb), white blood
cell count (WBC), lysozyme, nitroblue tetrazolium (NBT), total serum protein, albumin, globulin and albuminglobulin
ratio. Subsequently the treated fish were subjected to challenge test with Aeromonas hydrophila. In the
present study, primary stress markers were noticeably (p < 0.01) elevated with temperature stress and levels
were reduced with nutritional supplementation of TRP. Similarly, immuno-hematological parameters were altered
with the exposure of temeparture stress and got improved with dietary TRP supplementation. Results of the
present study suggest that dietary supplementation of 1.44% tryptophan has definitive role in the mitigation of
temperature stress and gives protection against bacterial infection to L. rohita.
Description
Keywords
Citation
J. Thermal Biol., 74:55-62