Functional and molecular characterization of a lipopeptide surfactant from the marine sponge-associated eubacteria bacillus licheniformis NIOT-AMKV06 of Andaman and Nicobar islands, India

Simple item page
dc.contributor.authorLawrance, A.
dc.contributor.authorBalakrishnan, M.
dc.contributor.authorJoseph, T.C.
dc.contributor.authorSukumaran, D.P.
dc.contributor.authorValsalan, V.N.
dc.contributor.authorGopal, D.
dc.contributor.authorRamalingam, K.
dc.date.accessioned2018-01-10T10:25:29Z
dc.date.available2018-01-10T10:25:29Z
dc.date.issued2014
dc.description.abstractThe production of a lipopeptide surfactant from the sponge-associated eubacteria Bacillus licheniformis NIOT-AMKV06 from the Andaman and Nicobar Islands was investigated. The highest production was attained with glucose and yeast extracts as the carbon and nitrogen sources (1.789 mg mL-1), respectively. The surfactant was highly stable over a pH range of 5.0–10 and a temperature range of 20–70 0C with high NaCl concentrations. Excellent emulsification activity was exhibited by the purified surfactant with crude oil, kerosene, and diesel. A two-fold increase in surfactant production (3.0 mg mL-1) was observed using the newly formulated medium in this study. The surfactant biosynthesis gene cluster (sfp, sfpO, and srfA) from B. licheniformis NIOT-AMKV06 was heterologously expressed in Escherichia coli, and the production was increased three-fold (11.78 gL-1) over the original strain. The results confirm the potential of the surfactant for use in bioremediation of hydrocarbons in a marine environment and for enhanced oil recovery. To our knowledge, this is the first report on the ability of a hydrocarbon degrading B. licheniformis from marine sponges for the biosynthesis of a potent lipopeptide surfactant possessing characteristics of maximum stability, outstanding surfactant activity, and exceptional emulsifying capability.en_US
dc.identifier.citationMarine Pollut. Bull. 82:76-85en_US
dc.identifier.urihttp://hdl.handle.net/123456789/3321
dc.language.isoenen_US
dc.publisherElsevier
dc.subjectAndaman and Nicobar Islandsen_US
dc.subjectMarine spongeen_US
dc.subjectBacillus licheniformisen_US
dc.subjectSurfactanten_US
dc.subjectSurfactant biosynthesis genesen_US
dc.titleFunctional and molecular characterization of a lipopeptide surfactant from the marine sponge-associated eubacteria bacillus licheniformis NIOT-AMKV06 of Andaman and Nicobar islands, Indiaen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
functional and molecular characterization of a lipopeptide.pdf
Size:
1.56 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Peer Reviewed Journal Articles (Inter.) (M,F&B)