A medium for enumeration of bacteria in foods containing swarming Bacillus spp.
A medium for enumeration of bacteria in foods containing swarming Bacillus spp.
Date
1993
Authors
Thampuran, Nirmala
Surendran, P.K
Journal Title
Journal ISSN
Volume Title
Publisher
Blackwell Publishing.
Abstract
Swarming of Bacillus spp. on agar plates may interfere with enumeration or isolation of bacteria in pickled, canned or fried fish products. A new medium containing sodium tripolyphosphate, EDTA disodium salt, dihydrate, sodium desoxycholate, supplemented with minerals and nutrient sources was developed, which was effective in inhibiting swarming of Bacillus spp. This medium polyphosphate-EDTA-desoxycholate agar (PEDA) was compared with tryptone glucose extract agar (Oxoid) for determining total plate count of various semi-conserved fishery products. While promoting discrete colony formation of Bacillus spp., PEDA provided good bacterial recovery. PEDA is recommended for isolation or enumeration of bacteria in [sea foods] dominated by swarming Bacillus spp.
Description
Keywords
Bacteria, Media, Microbiological techniques, Sea food
Citation
Letters in Applied Microbiology 1993: 16(5), 277-279