A High Performance Liquid Chromatographic Method for Quantitative Determination of Tetracyclines and its Epimers in Shrimp Muscle
A High Performance Liquid Chromatographic Method for Quantitative Determination of Tetracyclines and its Epimers in Shrimp Muscle
Date
2013
Authors
Thomas, Shiny
Priya, Lakshmi
Prasanth, K. P.
Nair, P. G. Viswanathan
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Fisheries Technologists (India)Cochin
Abstract
A method for determination of tetracycline, oxytetracycline,
chlortetracycline, 4-epitetracycline, 4-
epioxytetracycline and 4-epichlortetracycline in
shrimp muscle tissue by high performance liquid
chromatographic method (HPLC) with a UV
detector is described. The method involves extraction
of the analytes by McIlvaine buffer, clean up
by C18 SPE cartridge and analysis by HPLC and UV
detection. A good separation of all the six analytes
was achieved and good recovery (58 to 89%),
repeatability and within laboratory reproducibility
were possible with this method.
Description
Keywords
HPLC method, tetracycline, oxytetracycline, chlortetracycline, tetracycline epimers, shrimp muscle
Citation
Fishery Tech 50(1):75-79