Genotypes and phenotypes of methicillin-resistant staphylococci isolated from shrimp aquaculture farms

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Environ Microbiol Rep - 2021 - Rajan - Genotypes and phenotypes of methicillin‐resistant staphylococci isolated from shrimp.pdf(417.16 KB)
Date
2021-07-19
Authors
Rajan, Vineeth
G.K., Sivaraman
Vijayan, Ardhra
Elangovan, Ravikrishnan
Prendiville, Alison
Till T. Bachmann
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley
Abstract
The population of methicillin-resistant (MR) staphylococci in aquatic environment is rarely investigated. Here, we characterized a collection of MR staphylococci recovered from shrimp aquaculture farms (n = 37) in Kerala, India. A total of 261 samples yielded 47 MR isolates (16 S. aureus, 13S. haemolyticus, 11S. epidermidis, 3 S. saprophytics and 2 each of S.intermedius and S. kloosii). Multi-drug resistance was evident in 72.3% of the isolates, with resistance mainly towards erythromycin (78.7%), norfloxacin and trimethoprim-sulfamethoxazole (53.2%), and gentamicin (34%). Major resistance genes identified included mecA (100%), ermC (38.3%), aacA-aphD (21.3%), tetK (14.9%) and tetM (21.3%). Almost 60% of the isolates carried type V SCCmec (Staphylococcal Cassette Chromosome mec), and the remaining harboured untypeable SCCmec elements. Comprehensive genotyping of the methicillin-resistant Staphylococcus aureus isolates revealed high prevalence of ST772-t345-V (sequence type-spa type-SCCmec type) (75%), followed by minor representations of ST6657-t345-V and ST3190-t12353. The isolates of S. haemolyticus and S. epidermidis were genotypically diverse as shown by their pulsed-field gel electrophoresis (PFGE) profiles. Genes encoding staphylococcal enterotoxins were observed in 53.2% of the isolates. Various genes involved in adhesion and biofilm formation were also identified. In conclusion, our findings provide evidence that shrimp aquaculture settings can act as reservoirs of methicillin-resistant staphylococci.
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Environmental Microbiology Reports (2022) 14(3), 391–399
URI
https://drs.cift.res.in/123456789/6576
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Peer Reviewed Journal Articles (Inter.) (M,F&B)