Culture-independent real-time PCR assay for rapid enumeration of salmonella in seafood

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dc.contributor.authorKumar, R.
dc.contributor.authorSurendran, P.K.
dc.contributor.authorThampuran, N.
dc.contributor.authorLalitha, K.V.
dc.date.accessioned2014-02-22T06:26:07Z
dc.date.available2014-02-22T06:26:07Z
dc.date.issued2010
dc.description.abstractSalmonella is most important food-borne pathogen and commonly associated with water and food-borne outbreaks. Approximately 1 .4 million cases of salmonellosis were reported in the USA each year and similarly, outbreaks were reported in the Asian and European countries (D'Aoust, 1989). The incidence of salmonellosis over a period (1988-1992) in USA showed the 7.4% outbreaks were associated with the consumption of fish or shellfish (Bean et al., 1997). Prevalence of Salmonella contamination in seafood in imported and internal market of the United States was reported in a ten year study from 1990 to 1998 by field laboratories of USFDA and demonstrated that 11312 imported (7.1%) and 768 domestic (1.3%) seafood samples were positive for Salmonella (Heinitz et al., 2000). The contaminations in the coastal areas and the post harvest handling in seafood have been found responsible for Salmonella contamination in fish and seafood (Hatha and Lakshmanaperumalsamy, 1997; Kumar et al., 2009). There is a report on influence of environmental factors and human activity on the presence of Salmonella serovars in the marine environments of Galicia region in Spain (Marti nez-Urtaza et al., 2004). In most of the cases, Salmonella load in food and feed samples are reported to be low in numbers (Seo et al., 2006; Boughton et al., 2007). In such cases, sometime culture method may not be able to detect the presence of Salmonella. Thus, it is important to quantify the presence of contaminant in food items so as to establish the quantitative microbial risk assessment measures.en_US
dc.identifier.citationProceedings of the National Seminar on Conservation and Sustainability of Coastal Living Resources of India, 1-3 December 2009, Cochin, ed.by Meenakumari, B. et.al 606-615en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1583
dc.language.isoenen_US
dc.publisherSociety of Fisheries Technologists (India)en_US
dc.subjectPCRen_US
dc.subjectsalmonellaen_US
dc.subjectseafooden_US
dc.subjectIndian mackerelen_US
dc.subjectshrimp samplesen_US
dc.subjectchromagar methoden_US
dc.titleCulture-independent real-time PCR assay for rapid enumeration of salmonella in seafooden_US
dc.typeArticleen_US
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