Influence of vitamin C on hematololy of Pangasianodon hypophthalmus (Sauvage, 1878) juveniles during pre and post-challenge with Aeromonas hydrophila (Chester, 1901)
Influence of vitamin C on hematololy of Pangasianodon hypophthalmus (Sauvage, 1878) juveniles during pre and post-challenge with Aeromonas hydrophila (Chester, 1901)
Date
2018
Authors
Daniel, N.
Muralidhar, A.P.
Srivastava, P.P.
Jain, K.K.
Prasad, K.P.
Anandan, R.
Manish, J.
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Fisheries Technologists (India), Cochin
Abstract
A 60-day feeding trial was conducted to know the
effect of vitamin C on the hematological responses
of Pangasianodon hypophthalmus juveniles during
pre- and post-challenge conditions with Aeromonas
hydrophila. A total of seven purified diets were
prepared with 0 (control), 17.5 (T1), 35 (T2), 70 (T3),
175 (T4), 350 (T5) and 700 (T6) mg ascorbic acid
(AA) equivalent per kg of diet, supplied as Lascorbyl-
2-polyphosphate (LAPP) and fed to triplicate
group of P. hypophthalmus juveniles (mean
initial body weight: 3.23±0.01 g to 3.38±0.01 g) for
60 days twice daily. After the feeding and after
challenging with A. hydrophila, the haematological
parameters were measured in P. hypophthalmus
juveniles. All haematological parameter values
(except albumin-globulin ratio) viz., serum glucose,
total protein, albumin, globulin, hemoglobin (Hb),
total erythrocyte count (TEC), total white blood cells
count (WBC), hematocrit value (HCT) increased
significantly (p<0.05) in treatment groups than
control (vitamin C depleted diet). Further, the
increased hematological values for most of the
parameters were observed in the range of 175 to 350
mg AA kg-1. Therefore, it is suggested that dietary
supplementation of 175 to 350 mg AA kg-1 diet
would be beneficial for the optimal hematological
responses for P. hypophthalmus juveniles
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Citation
Fishery Technology 55(2):120-127