Haemolytic studies on Fish, Sheep and Human Blood Agar against Pathogenic Bacteria
Haemolytic studies on Fish, Sheep and Human Blood Agar against Pathogenic Bacteria
Date
2020
Authors
Nadella, Ranjit Kumar
V. A, Minimol
Nilavan, Ezhil
Muthulakshmi, T.
Basha, K. Ahamed
Prasad, M. M.
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Fisheries Technologists (India)
Abstract
In this study, β-haemolytic activity of fish blood
(Cyprinus rubrofuscus), sheep blood (SB) and human
blood (HB) was assessed for studying haemolysis
against reference bacterial strains Edwardsiella tarda
(ATCC 15947); Aeromonas hydrophila (ATCC 35654);
Enterobacter cloacae (ATCC 13047); Pseudomonas
aeruginosa (ATCC 10145); Escherichia coli (ATCC
35218); Staphylococcus aureus (ATCC 43300); Vibrio
cholera (MTCC 3906) and Salmonella paratyphii
(ATCC 15305). Both human blood and fish blood
showed similar β-haemolytic activity for all pathogens
except E. cloacae on fish blood. The zone of
haemolysis (mm) recorded in FB is 18, 28, 0, 22, 25,
14, 20, 22; in HB is 13, 15, 14, 23, 17, 18, 15, 17 and
in SB is 14.6, 13.8, 15.8, 26.2, 19.4, 21.2, 17.6, 16.2
after 48 h respectively for E. tarda; A. hydrophila; E.
cloacae; P. aeruginosa; E. coli; S. aureus; V. cholera and
S. paratyphii. The present study also reports the
antimicrobial property of fish blood against the
commonly occurring fish pathogens. The result
indicated a decrease in optical density readings in
fish blood supplemented broth compared to control
broth (without blood). This indicates clear inhibition
of growth for the three pathogens in BHI broth
supplemented with fish blood possesed due to the
presence of antimicrobial substances in the fish
blood. In conclusion, β-haemolytic activity observed
better in FB plates when compared with HB plates
and therefore, fish blood can be used as an
alternative to sheep blood for haemolytic studies of
fish pathogens.
Description
Keywords
Haemolysis, Fish blood, Pathogens, Antibacterial activity