Changes in Electrophoretic patterns of Sarcoplasmic Proteins and Myofibrillar Proteins in Caranx melampygus (Cuvier, 1833) during chilled storage
Changes in Electrophoretic patterns of Sarcoplasmic Proteins and Myofibrillar Proteins in Caranx melampygus (Cuvier, 1833) during chilled storage
Date
2020
Authors
Hassan, Femeena
Nija, K. V.
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Fisheries Technologists (India)
Abstract
SDS-PAGE studies were conducted to apprehend
the changes in the electrophoretic pattern of
myofibrillar and sarcoplasmic proteins of Caranx
melampygus (Cuvier, 1833) (Blue fin Trevally) during
chilled storage. The study revealed that the intensity
of Myosin bands were getting reduced during
storage. The relative front of myosin band was
getting decreased during storage.The relative front
on initial day for myosin is 0.331 and it decreased
to 0.303 on 12thday of storage. In protein profile,
molecular weights of protein bands were found to
be 191.8kDa, 99.1 kDa, 52.0 kDa for myofibrillar
proteins on initial day, and 200.0 kDa, 101.4 kDa,
51.5 kDa on 12th day. Molecular weights of protein
bands were found to be 97.4 kDa, 60.3 kDa, 50.9 kDa,
30.4 kDa, 29.2 kDa, 26.8 kDa, 25.6 kDa, 21.5 kDa for
sarcoplasmic proteins on initial day and 97.4 kDa,
61.1 kDa, 52.1 kDa, 45.8 kDa, 34.6 kDa, 30.0 kDa,
26.1 kDa ,21.5 kDa respectively on 12th day. The
number of bands for sarcoplasmic proteins and
myofibrillar proteins of Caranx melampygus were
same on initial day and final day of storage (9 and
3 respectively).
Description
Keywords
SDS- PAGE, salt soluble protein, water soluble protein