Characterization of tailed phages against E. coli using DNA restriction digestion analysis
Characterization of tailed phages against E. coli using DNA restriction digestion analysis
Date
2021
Authors
Karthika, R.
George, Iris
Murugadas, V.
Jeswin, J.
Sherin, P. S. Anna
Sanjeev, Devi
Visnuvinayagam, S.
Manikanta, B.
Prasad, M. M.
Rao, B. Madhusudana
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Fisheries Technologists (India)
Abstract
In the context of emerging newer forms of antimicrobial
resistance multidrug resistance (MDR),
extensively drug resistance (XDR) and pan drug
resistance (PDR) in pathogens, bacteriophages remain
a promising alternative antimicrobial weapon
to control it (Falagas & Karageorgopoulos, 2008,
Magiorakos et al., 2012, Ghajavand et al., 2017).
Bacteriophages are viruses that infect and kill
bacteria and have many advantages when used
alone or in combination with antibiotics viz., host
specificity, self-limiting replications, harmlessness to
the native microflora, longer period for resistance
development, are significantly safer and are better
tolerated without infecting mammalian cells (Principi
et al., 2019). Bacteriophages can disperse a bacterial
biofilm, which is very difficult to eradicate with
standard antibiotic therapy (Lu & Collins, 2007).
Bacteriophages enter the host bacteria through the
receptors, replicate inside the host with the host
cellular machinery and enter either lytic or lysogenic
mode (Ofir & Sorek, 2018). Owing to these unique
characteristics, bacteriophages are being freshly
evaluated taxonomically (Sharma et al., 2017).
Various aquatic environments such as sewage,
canals, rivers, lakes, mangroves, coastal waters,
aquaculture ponds, etc. remain a sink for the
presence of diverse bacteriophages with variations
in the host range of activities (Flu & Flu, 1947; Jin
et al., 2019; Topka et al., 2019).
Description
Keywords
tailed phages, E. coli, DNA restriction