Thiamine and Pyridoxine loaded Vanillic acid Grafted Chitosan: A Functional Food ingredient to mitigate swimming induced Oxidative stress in Animal model
Thiamine and Pyridoxine loaded Vanillic acid Grafted Chitosan: A Functional Food ingredient to mitigate swimming induced Oxidative stress in Animal model
Date
2021
Authors
Tejpal, C. S.
Elavarasan, K.
Lekshmi, R. G. K.
Chatterjee, N. S.
Anuj, K.
Anas, K. K.
Devi, H. M
Sanal, E.
Anandan, R.
Mathew, S.
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Fisheries Technologists (India)
Abstract
The study was conducted to evaluate the biofunctional
properties of thiamine and pyridoxineloaded
vanillic acid grafted-chitosan (TPVGC) to
perform as an oxidative stress mitigating agent. The
antioxidant properties of TPVGC assessed in terms
of DPPH and ferric reducing antioxidant power
(FRAP) assay indicated that the bioactivities are
mainly dose dependent. Further, the metabolic
enzyme associated beneficial properties of TPVGC
were assessed in an animal model through swimming
induced stress. Animals were randomly
distributed into four experimental groups and fed
with TPVGC at 0, 0.8, 1.6 and 2.4% in the diet for
a period of 45 days. During the experimental period,
the animals were exposed to swimming exercise for
50 min daily. Average swimming-time and antifatigue
activity revealed that the animals fed with
graded level of TPVGC enhanced stamina and
swimming activity. Control group fed without
TPVGC were lethargic after 30 min of swimming.
Metabolic responses were assessed in terms of
lactate dehydrogenase (LDH), malate dehydrogenase
(MDH), catalase, superoxide dismutase (SOD)
and acetylcholine esterase (AChE) activity. LDH,
MDH, catalase and SOD activities of treatment
group showed significant (p<0.05) reduction compared
to control. However, a reverse trend was
observed for AChE activity. This was further
supported by the results of Principal Component
Analysis (PCA) which indicates the major variables contributed to variation in case of individual tissue
samples. Based on the results, it is inferred that the
supplementation of TPVGC has stress mitigation
role along with enhanced stamina during the
swimming exercise. It is hence surmised that
TPVGC can function as a potential nutraceutical due
to its proven functional properties notably faster
stress recovery and stamina boost, which has
prospective applications in human healthcare/livestock
segments in the contemporary global scenario.
Description
Keywords
Antioxidant properties, Swimming exercise, Pyridoxine