Characterization of a Bioactive Protein with Antimicrobial Property from Loligo sp.
Characterization of a Bioactive Protein with Antimicrobial Property from Loligo sp.
Date
2014
Authors
Cikesh, P.C
Karthikeyan, P
Bindiya, E.S
Subin, S. Raghul
Tina, K. J.
Chandrasekaran, M
Bhat, Sarita G.
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Fisheries Technologists (India)Cochin
Abstract
Loligo is a genus of squids. Due to their abundance
and economic importance in the export market,
squids have been studied for their biology and
fishery characteristics. The aim of the current study
was to investigate anti-microbial activity associated
with proteins extracted from this organism. Whole
body tissue was homogenized with protein extraction
buffer and its antimicrobial activity was
screened against Klebsiella pneumoniae, Bacillus cereus,
Salmonella typhimurium, Escherichia coli, Staphylococcus
aureus, Pseudomonas aeruginosa and Vibrio cholerae
by agar well diffusion method. The protein was
concentrated by acetone precipitation. Purification
was done in different steps employing cut off
membrane filtration, thin layer chromatography
(TLC) and Sephadex G-50 gel filtration column. The
molecular weight of the protein was determined by
SDS-PAGE. A 45 kDa protein was isolated and
purified from Loligo with a total yield of 0.06%
which showed bioactivity against V. cholerae. MALDI
and MASCOT analysis showed that the 45 kDa
protein had a similarity score of 40 with parvalbumin
protein of Fundulus similis.
Description
Keywords
Antimicrobial activity, Vibrio cholerae, Sepahdex G-50, SDS-PAGE, MALDI, MASCOT
Citation
Fishery Techn 51(4):247-251