Frequency and Expression of Antibiotic Resistance in Luminous Vibrio harveyi

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Date
2005
Authors
Abraham, T. Jawahar
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Fisheries Technologists (India)Cochin
Abstract
In-vitro activities of chloramphenicoi, ciprofloxacin, nalidixic acid and oxytetra-cycline were evaluated against penaeid shrimp larval pathogen, luminous Vibrio liarveyi. The frequency at which three strains of luminous V. harveyi strains can mutate to develop resistance to these antibiotics was also estimated. Resistant mutants developed at lower frequencies to chloramphenicol (<9.43 x lO"10) and ciprofloxacin (<1.14 x lO"10 - 1.61 x lO"9) than to nalidixic acid (6.61 x lO"5 - 8.87 x lO"5) and oxytetracycline (6.82 x lO"6 - 4.74 x lO"4) at 10 times the respective minimal inhibitory concentration (MIC). Low concentration of antibiotics (5 x MIC and 2 x MIC), however, induced more of mutant strains. Although ciprofloxacin was effective in terms of MIC and induction of low level of mutants, chloramphenicol was more effective in its ability to kill both resistant and sensitive strains of luminous V. harveyi
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Keywords
Vibrio harveyi, antibiotic resistance, mutation frequency, shrimp hatchery
Citation
Fishery Tech 42(2):197-202
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