Studies on Salmonella Isolates from Fishery Products
Studies on Salmonella Isolates from Fishery Products
Date
2005
Authors
Shashidhar, R
Jajoo, S
Karani, M
Warrier, S.B
Bandekar, J.R
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Fisheries Technologists (India)Cochin
Abstract
Salmonella isolates obtained from fishery products were studied with respect to their
sensitivity to antibiotics, ionizing radiation and chlorine water treatment. The optimum pH
range for the growth of the isolates was determined. The isolates were also characterized
for pathogenicity related genes based on PCR assay. None of the 34 isolates under study
were resistant to 16 antibiotics tested. All isolates were sensitive to chlorine water treatment
(25 ppm) when treated in saline, but they were resistant when treatment was in organic
medium. Optimum growth of all isolates was observed in the pH range of 5 to 7; however,
S. typhimurium and S. worthington could grow even at pH 4. The PCR based identification
of pathogenicity related genes invA and spvC showed that both genes were present in S.
gallinamm and S. enteritidis and only invA was present in S. typhimurium and S. worthington.
D
10 value for these serovars irradiated in brain heart infusion broth was in the range of 270
to 289 Gy.
Description
Keywords
Antibiotic resistance, chorine water, ionizing radiation. Salmonella, PCR.
Citation
Fishery Tech 42(2):217-224