Antioxidant and hepatoprotective property of squalene for counteracting the oxidative damage induced by methotrexate in experimental rats
Antioxidant and hepatoprotective property of squalene for counteracting the oxidative damage induced by methotrexate in experimental rats
Date
2020
Authors
Sumi, Edakkukaran Sudhakaran
Dara, Pavan Kumar
Mannuthy, Rosemol Jacob
Ganesan, Balaraman
Anandan, Rangasamy
Mathew, Suseela
Journal Title
Journal ISSN
Volume Title
Publisher
Acta Biologica Szegediensis
Abstract
Methotrexate (MTX), an antifolate drug, is extensively prescribed for
patients suff ering from diseases like cancer, psoriasis, neoplasms, and rheumatoid
arthritis. Despite its eff ectiveness, MTX sometimes fi nds limited application because its
undesirable side eff ects, including hepatic or renal impairment, bone marrow toxicity and
gastrointestinal mucosal injury. Squalene, a highly unsaturated isoprenoid compound,
isolated from shark liver oil has great potential in neutralizing the damaging eff ects triggered
by free radicals. Therefore, in this study, the protective role of dietary squalene
supplementation on oxidative stress induced by methotrexate in experimental rats
was evaluated. A signifi cant reduction was displayed in the activities of catalase (CAT)
and superoxide dismutase (SOD) in MTX-intoxicated groups compared to other groups.
Similarly, the activities of glutathione dependant enzymes (GPx and GST) and reduced
glutathione (GSH) in MTX-induced groups were shown to be lower compared to the
untreated control. Increased LPO (lipid peroxide) level was found in MTX-intoxicated
groups compared to other groups. In addition, alterations in the levels of liver marker
enzymes like AST, ALP, ALT, and LDH were noticed in MTX intoxicated groups compared
to other groups. Biochemical results were confi rmed by the histopathological examination
of liver sections. In conclusion, the result obtained in the present study proposes
that squalene exerts antioxidant activity and is capable of ameliorating oxidative stress
and liver injury induced by MTX.
Description
Keywords
Hepatotoxicity, Histopathology, Methotrexate, Squalene
Citation
Volume 64(2):199-206, 2020, http://abs.bibl.u-szeged.hu/index.php/abs