FT Vol.56(4)

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Browsing FT Vol.56(4) by Author "Panda, S.K."

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    Chilled storage study of value added black clam (Villorita cyprinoides) (Gray, 1825) products in two different packaging materials
    (Society of Fisheries Technologists (India), Cochin, 2019) Anju, A.V.; Harsha, K.; Sethulakshmi, C.S.; Sreejith, S.; Panda, S.K.; Gopal, N.; Bindu, J.
    Black clam (Villorita cyprinoides) (Gray, 1825), a nutrient rich bivalve was used for preparing two products viz., clam cutlet and clam bonda, and packaged in two different packaging materials, 200 gauge low density polyethylene and 12 μ polyester/ 200 gauge polyethylene laminate. The biochemical and microbiological parameters of the products were analyzed to study its storage stability in chilled condition. Parameters like pH, color (L*, a*, b*), total volatile base nitrogen (TVBN), thiobarbituric acid (TBA), peroxide value (PV) and free fatty acid (FFA) were within permissible limit during the period of observation. The aerobic plate count, after 24 days of storage of the products were in the range of 6.81 to 7.41 log10 cfu g-1 and 6.3 to 7 log10 cfu g-1 for LDPE and PES/PE respectively. Escherichia coli and Enterobacteriaceae were absent throughout the storage period. The study showed that biochemical and microbiological parameters were greater for the products packed in LDPE compared to PES/PE laminate, indicating that the laminate film was more efficient in preserving the quality of the products when compared to monolayer films.
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    Comparative analysis of RAPD, RFLP and PFGE for the characterization of pathogenic vibrio parahaemolyticus isolated from seafood and coastal environment
    (Society of Fisheries Technologists (India), Cochin, 2019) Minimol, V.A.; Kishore, P.; Nadella, R.K.; Panda, S.K.; Nayak, B.B.; Prasad, M.M.; Kumar, H.S.
    Vibrio parahaemolyticus is ubiquitous in coastal marine environment. Certain strains of V. parahaemolyticus are pathogenic to humans and harbors either or both thermostable direct hemolysin (tdh) gene and tdh related hemolysin (trh) gene. Pathogenic V. parahaemolyticus isolates from the aquatic environment from Mumbai, India were characterized with respect to their virulence genotypes by employing random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) and pulsed field gel electrophoresis (PFGE). Out of 140 samples, twenty five samples harboured virulent V. parahaemolyticus. Pandemic group specific PCR assays viz., GSVP, GSOLD, GSOK, ORF8 and PGS PCR showed differential performances which revealed difficulties in the confirmation of pandemic clone from environmental samples. Results from the molecular characterization employing RAPD and RFLP indicated that the PGS PCR positive isolates (2) showed identical banding pattern in RFLP analysis, whereas GS:OK positive isolates (4) showed similar RAPD banding pattern with 80% similarity among 11 pathogenic strains studied. However, in whole genome comparison by PFGE using notI restriction enzyme, these isolates were scattered in different groups at 40% similarity level. This study suggests that the genetically diverse pathogenic strains of V. parahaemolyticus with characteristic of pandemic clones are present in the coastal environment.