FT Vol.56(4)

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    Comparative analysis of RAPD, RFLP and PFGE for the characterization of pathogenic vibrio parahaemolyticus isolated from seafood and coastal environment
    (Society of Fisheries Technologists (India), Cochin, 2019) Minimol, V.A.; Kishore, P.; Nadella, R.K.; Panda, S.K.; Nayak, B.B.; Prasad, M.M.; Kumar, H.S.
    Vibrio parahaemolyticus is ubiquitous in coastal marine environment. Certain strains of V. parahaemolyticus are pathogenic to humans and harbors either or both thermostable direct hemolysin (tdh) gene and tdh related hemolysin (trh) gene. Pathogenic V. parahaemolyticus isolates from the aquatic environment from Mumbai, India were characterized with respect to their virulence genotypes by employing random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) and pulsed field gel electrophoresis (PFGE). Out of 140 samples, twenty five samples harboured virulent V. parahaemolyticus. Pandemic group specific PCR assays viz., GSVP, GSOLD, GSOK, ORF8 and PGS PCR showed differential performances which revealed difficulties in the confirmation of pandemic clone from environmental samples. Results from the molecular characterization employing RAPD and RFLP indicated that the PGS PCR positive isolates (2) showed identical banding pattern in RFLP analysis, whereas GS:OK positive isolates (4) showed similar RAPD banding pattern with 80% similarity among 11 pathogenic strains studied. However, in whole genome comparison by PFGE using notI restriction enzyme, these isolates were scattered in different groups at 40% similarity level. This study suggests that the genetically diverse pathogenic strains of V. parahaemolyticus with characteristic of pandemic clones are present in the coastal environment.
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    Effect of Plectranthus amboinicus leaf extract on the quality attributes of microencapsulated fish oil fortified soup powder
    (Society of Fisheries Technologists (India), Cochin, 2019) Kumar, L.R.G.; Jayathilakan, K.; Sarika, K.; Priya, E.R.; Greeshma, S.S.; Sultana, K.; Tejpal, C.S.; Mathew, S.
    The effect of Plectranthus amboinicus leaf extract (PAE) (phenolic content of 2559.56 mg gallic acid equivalents/L) addition in a microencapsulated fish oil fortified soup powder was evaluated in the present study. Fortification of PAE significantly improved the color, rehydration and water activity of the soup powder. The oxidative indices unveiled the role of PEA in protecting the MFO fortified soup powder from oxidative deterioration during the storage period. No faecal coliforms, yeast and mould growth was observed in MFO and PAE fortified soup powder whereas mould growth was there in the control samples. The fortified soup powder has scored high in terms of aroma, taste, consistency and overall acceptability proving its sensory acceptance. The significant findings of the storage study clearly depicted the antioxidant, antibacterial, antifungal as well as flavour enhancing potential of PAE and hence it can be recommended as a potent natural preservative for fish oil incorporated food systems.
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    A preliminary study on trawl geometry: Effect of speed and warp length on mouth opening
    (Society of Fisheries Technologists (India), Cochin, 2019) Jha, P.N.; Chinnadurai, S.; Renjith, R.K.; Madhu, V.R.; Soni, J.
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    A review on techniques and challenges in the harvest of mesopelagics
    (Society of Fisheries Technologists (India), Cochin, 2019) Remesan, M.P.; Prakash, R.R.; Prajith, K.K.; Jha, P.N.; Renjith, R.K.; Boopendranath, M.R.
    Harvesting of underutilized mesopelagic resources is among the possible solutions to increase capture fish production, which will also facilitate recovery of overexploited fish resources. Mesopelagics can be a source for fish meal, which is in great demand from the growing aquaculture and animal feed industry and will find applications in areas like nutraceutical and cosmetic industries. Recent estimates based on acoustic data, put the biomass of mesopelagic fishes between 11,000 and 15,000 million tonnes in the world oceans. Even though there is a large potential for developing a fishery for mesopelagics, very little information is available on the appropriate commercial harvesting systems. About 75% of total global catch of small mesopelagic fishes is accounted by myctophids. Average world annual production of myctophids between 1970 and 2015 has been 10,640 tonnes, with a maximum of 74,751 tonnes in 1990. Aimed midwater trawling and pair trawling with appropriate trawl designs have been considered suitable for harvesting mesopelagic resources. An overview of the study is the mesopelagics in the Indian Ocean and its availability in the deep sea shrimp trawl bycatch. Sampling gears and commercial fishing techniques targeting mesopelagics are discussed along with details of experimental aimed midwater trawling conducted in Arabian Sea, targeting mesopelagic resources.