Peer Reviewed Journal Articles (Inter.) (M,F&B)

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Browsing Peer Reviewed Journal Articles (Inter.) (M,F&B) by Author "Bansal, K.C."

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    Characterization and phylogenitic analaysis of ecotine biosynthesis genes from bacillus halodurans
    (Springer, 2008) Rajan, A.; Joseph, C.T.; Thampuran, N.; James, R.; Viswanathan, C.; Bansal, K.C.
    Ectoine, a cyclic tetrahydropyrimidine (2- methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid), is a natural compound, which serves as a protective substance in many bacterial cells. In this study, the putative ectABC gene cluster from Bacillus halodurans was heterologously expressed in E. coli and the production of ectoine was con- Wrmed by HPLC analysis. The activity of the enzymes coded by the ectA, B and C genes were found to be higher in induced transgenic cells compared to the uninduced cells. Phylogenetic analysis revealed sequence identities ranging from 36–73% for ectA gene, 55–81% for ectB gene and 55–80% for ectC gene indicating that the enzymes are evolutionarily well conserved.