Characterization and phylogenitic analaysis of ecotine biosynthesis genes from bacillus halodurans
Characterization and phylogenitic analaysis of ecotine biosynthesis genes from bacillus halodurans
Date
2008
Authors
Rajan, A.
Joseph, C.T.
Thampuran, N.
James, R.
Viswanathan, C.
Bansal, K.C.
Journal Title
Journal ISSN
Volume Title
Publisher
Springer
Abstract
Ectoine, a cyclic tetrahydropyrimidine (2-
methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid), is
a natural compound, which serves as a protective substance
in many bacterial cells. In this study, the putative ectABC
gene cluster from Bacillus halodurans was heterologously
expressed in E. coli and the production of ectoine was con-
Wrmed by HPLC analysis. The activity of the enzymes
coded by the ectA, B and C genes were found to be higher
in induced transgenic cells compared to the uninduced
cells. Phylogenetic analysis revealed sequence identities
ranging from 36–73% for ectA gene, 55–81% for ectB gene
and 55–80% for ectC gene indicating that the enzymes are
evolutionarily well conserved.
Description
Keywords
B. halodurans, compatible solutes, salt stress, ectoine genes, osmoregulation
Citation
Archives of Microbiology 2008: 190, 481-487