Characterization and phylogenitic analaysis of ecotine biosynthesis genes from bacillus halodurans

dc.contributor.author	Rajan, A.
dc.contributor.author	Joseph, C.T.
dc.contributor.author	Thampuran, N.
dc.contributor.author	James, R.
dc.contributor.author	Viswanathan, C.
dc.contributor.author	Bansal, K.C.
dc.date.accessioned	2013-12-20T06:00:40Z
dc.date.available	2013-12-20T06:00:40Z
dc.date.issued	2008
dc.description.abstract	Ectoine, a cyclic tetrahydropyrimidine (2- methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid), is a natural compound, which serves as a protective substance in many bacterial cells. In this study, the putative ectABC gene cluster from Bacillus halodurans was heterologously expressed in E. coli and the production of ectoine was con- Wrmed by HPLC analysis. The activity of the enzymes coded by the ectA, B and C genes were found to be higher in induced transgenic cells compared to the uninduced cells. Phylogenetic analysis revealed sequence identities ranging from 36–73% for ectA gene, 55–81% for ectB gene and 55–80% for ectC gene indicating that the enzymes are evolutionarily well conserved.	en_US
dc.identifier.citation	Archives of Microbiology 2008: 190, 481-487	en_US
dc.identifier.uri	http://hdl.handle.net/123456789/1015
dc.language.iso	en	en_US
dc.publisher	Springer	en_US
dc.subject	B. halodurans	en_US
dc.subject	compatible solutes	en_US
dc.subject	salt stress	en_US
dc.subject	ectoine genes	en_US
dc.subject	osmoregulation	en_US
dc.title	Characterization and phylogenitic analaysis of ecotine biosynthesis genes from bacillus halodurans	en_US
dc.type	Article	en_US

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