2015

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Browsing 2015 by Author "Anandan, R."

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    Biochemical Composition and Heavy Metal Content of Selected Marine Fish from the Gulf of Mannar, India
    (Central Institute of Fisheries Technology, Cochin, India, 2015) Ajeeshkumar, K.K.; Visnu, K.V.; Remyakumari, K.R.; Navaneethan, R.; Asha, K.K.; Ganesan, B.; Chatterjee, N.S.; Anandan, R.; Suseela Mathew
    Biochemical composition of five marine fish; grey bamboo shark, yellow margin trigger fish, paleedged stingray, laced moray and Japanese leatherjacket fish from the Gulf of Mannar, India were evaluated. Moisture, protein, lipid and ash content for the samples ranged between 75.07-76.74, 17.99-20.97, 1-3.5 and 1.46-1.93% respectively. Profiles of heavy metal, fatty acid and amino acid data were also collected. The predominant fatty acids recorded in all five species were docosahexaenoic acid (DHA), arachidonic acid (AA) and palmitic acid. All five species contain high content of different essential and non-essential amino acids namely glutamic acid, aspartic acid, arginine, serine and lysine. Significant amount of iron (13-50 ppm), zinc (2.5-10.5 ppm) and copper (0.18-4.03 ppm) were found in all the fishes. Among heavy metals, cadmium (0.05-0.1 ppm) was detected which is below permissible limit.
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    Protective Effects of Echinorhinus brucus Liver Oil against Induced Inflammation and Ulceration in Rats
    (Central Institute of Fisheries Technology, Cochin, India, 2015) Vishnu, K.V.; Ajeeshkumar, K.K.; Asha, K.K.; Remyakumari, K.R.; Ganesan, B.; Anandan, R.; Chatterjee, N.S.; Suseela Mathew
    Anti-inflammatory and anti-ulcer activities of Bramble shark (Echinorhinus brucus) liver oil were examined in rats. The oil showed significant proportion of n-3 polyunsaturated fatty acids (PUFAs), the percentages of EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) being 16 and 18% respectively. The study also revealed that liver oil had a very favourable n3:n6 ratio of 4.7. Oral administration of shark liver oil at 1g kg-1 concentration significantly attenuated the formalin-induced paw edema in experimental rats. It exerted potent anti-ulcer effect against acid-ethanol mixturemediated lesion formation in the rat gastric mucosa.
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    Supercritical Carbon Dioxide Extraction of PUFA Rich Oil from Freeze Dried Tuna Red Meat
    (Central Institute of Fisheries Technology, Cochin, India, 2015) Yathavamoorthi, R.; Nithin, C.T.; Ananthanarayanan, T.R.; Suseela Mathew; Bindu, J.; Anandan, R.; Sreenivasagopal, T. K.
    Supercritical fluid extraction (SFE) is an emerging technology for extraction and isolation of valuable compounds from natural products. Supercritical carbon dioxide (SCO2 ) is one of the most commonly used solvents in SFE and has gained importance as a “green” or environment friendly solvent. In this study, polyunsaturated fatty acid (PUFA) rich oil from freeze dried yellowfin tuna (Thunnus albacares) red meat was extracted using supercritical carbon dioxide. Red meat, a by-product obtained from tuna processing forms about 9-11% of the total body weight of tuna. Lipid extraction from freeze dried tuna meat was performed at a temperature of 60oC and pressure of 35 MPa for 3 h. The flow rate of CO2 was kept constant at 175 l h-1. The extracted oil was collected in two separators both held at 5 MPa pressure and temperature of 50oC and 40oC respectively. The antioxidant tocopherol (0.5%) was added to the extracted oil and stored at 2-4oC for further analysis. The yield of oil obtained was 5% and it was found to be rich in polyunsaturated fatty acids like docosahexaenoic, eicosapentaenoic and arachidonic acid constituting 31, 5 and 4% of the total fatty acids respectively. Palmitic and stearic acid were the most abundant saturated fatty acids present constituting 23 and 15% of total fatty acids respectively. Oleic acid contributed 18% of the total fatty acids. SFE was effective in extraction of fatty acids from tuna red meat with minimal losses.