FT Vol.52(3)

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    Biochemical Composition and Heavy Metal Content of Selected Marine Fish from the Gulf of Mannar, India
    (Central Institute of Fisheries Technology, Cochin, India, 2015) Ajeeshkumar, K.K.; Visnu, K.V.; Remyakumari, K.R.; Navaneethan, R.; Asha, K.K.; Ganesan, B.; Chatterjee, N.S.; Anandan, R.; Suseela Mathew
    Biochemical composition of five marine fish; grey bamboo shark, yellow margin trigger fish, paleedged stingray, laced moray and Japanese leatherjacket fish from the Gulf of Mannar, India were evaluated. Moisture, protein, lipid and ash content for the samples ranged between 75.07-76.74, 17.99-20.97, 1-3.5 and 1.46-1.93% respectively. Profiles of heavy metal, fatty acid and amino acid data were also collected. The predominant fatty acids recorded in all five species were docosahexaenoic acid (DHA), arachidonic acid (AA) and palmitic acid. All five species contain high content of different essential and non-essential amino acids namely glutamic acid, aspartic acid, arginine, serine and lysine. Significant amount of iron (13-50 ppm), zinc (2.5-10.5 ppm) and copper (0.18-4.03 ppm) were found in all the fishes. Among heavy metals, cadmium (0.05-0.1 ppm) was detected which is below permissible limit.
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    Microbiological Quality of Myctophid Fish of the Arabian Sea
    (Central Institute of Fisheries Technology, Cochin, India, 2015) Sanjoy, Das; Jose, Fernandez. T; Lalitha, K.V.
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    Characterization and Evaluation of Selected House-keeping Genes for Quantitative RT-PCR in Macrobrachium rosenbergii Morphotypes
    (Society of Fisheries Technologists (India)Cochin, 2015) Priyadarshi, Himanshu; Das, Rekha; Kumar, A. Pavan; P, Gireesh Babu; Javed, Hasan; Krishna, Gopal; Marappan, Makesh; Chaudhari, Aparna
    Macrobrachium rosenbergii (De Man, 1879) is an important freshwater prawn cultured globally and is known to exhibit three male morphotypes with differential growth rates. Several gene expression studies using qRT-PCR have been undertaken in this species using any one of the popularly used internal control genes. Here we evaluate four house-keeping genes namely EF1a, GAPDH, b-actin and 18S rRNA for transcript stability across various M. rosenbergii tissues in male morphotypes for use as internal control genes in expression studies. Expression of these genes was measured in 5 tissues (androgenic gland, gill, eyestalk, nerve cord and testis) across morphotypes and Ct values were analyzed using four statistical methods (DCt, geNorm, BestKeeper and NormFinder) developed for this purpose. We report that EF1a is the most suited internal control for data normalization in androgenic gland, testis, nerve cord and gills while GAPDH is best for eyestalk tissue of M. rosenbergii irrespective of the morphotype. Wherever two reference genes are required b-actin can be included for all the tissues except in nerve cord, where GAPDH is more suitable second reference gene. M. rosenbergii EF1a and GAPDH partial mRNA sequences were also generated as part of this study and their phylogenetic relation with other decapods is also reported.
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    Development of Ready to Drink Iron Fortified Shrimp Soup in Retortable Pouches
    (Central Institute of Fisheries Technology, Cochin, India, 2015) Shashidhar, K.; Biji, K.B.; Ravishankar, C.N.; Sreenivasagopal, T. K.; Jose, Joseph
    Soup prepared from Indian white shrimp (Fenneropenaeus indicus) was fortified with iron (0.024%) by incorporating sodium iron EDTA. The soup was thermal processed at F0 6.0, at three different temperatures viz., 110, 115 and 121.1oC in retortable pouches. All the samples were found to be commercially sterile. Iron fortification did not show any significant difference in sensory acceptance of the product after thermal processing at different temperatures. A higher b* value was observed in fortified soup compared to that of control soup. Thermally processed fortified shrimp soup was in acceptable condition even after 90 days at ambient temperature with slightly higher sensory scores for the product processed at 121.1°C.
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    Post-harvest Bacterial Quality of Lethrinus lentjan (Lacepede, 1802)
    (Central Institute of Fisheries Technology, Cochin, India, 2015) Femeena, Hassan; Treesa, G.; Geethalakshmi, V.; Sanjeev, S.
    Food safety and microbiological quality particularly in high moisture foods like seafood have gained significant attention among consumers. Compromise with regard to quality standards for the retail trade of fish and fish products is decreasing the quality of fish. Present study was conducted to detect the bacterial progression fish from harbour to retail shop and market. Lethrinus lentjan (white snapper) was collected from three different strategic locations, viz., harbour, retail shop and market on the same day and quantitative and qualitative bacteriological analyses were carried out. Samples collected from market exhibited 12.61% more mesophilic count than that of harbour samples and 9.5% more than retail samples. The increasing trend in bacterial counts was noticed in psychrophiles, enterobacteriaceae, faecal streptococci, hydrogen sulphide producers, Brochothrix thermosphacta and histamine producing bacteria. Pathogens like Salmonella, Vibrio cholerae, V. parahaemolyticus and Listeria monocytogenes were absent in the analysed samples.
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    Length-weight Relationship and Condition Factor of Cobia, Rachycentron canadum (Linnaeus, 1766) along Northwest Coast of India
    (Society of Fisheries Technologists (India)Cochin, 2015) Sajeevan, M.K; Kurup, B. Madhusoodana
    cobia breeds throughout the year with peak breeding season during July-August and October-December. Sajeevan (2011), through gonadosomatic index analysis and percentage of mature specimen, reported the peak breedingdifferent size groups, results obtained by all the three methods (Fulton’s condition factor, relative condition factor and modified condition factor) were almost complementary; indicating that cobia is in good condition throughout its life cycle
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    Length–weight Relationship of Myctophum spinosum (Steindachner, 1867) Caught off South-west Coast of India
    (Central Institute of Fisheries Technology, Cochin, India, 2015) Vipin, P.M.; Pradeep, K.; Jose Fernandez, T.; Madhu, V.R.; Ramesan, M.P.; Boopendranath, M.R.; Renju, Ravi
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    Effect of Monsoon on Sulphite Reducing Clostridia Levels in Fish from Retail Outlets in Maharashtra
    (Society of Fisheries Technologists (India) Cochin, 2015) Visnuvinayagam, S.; Viji, P.; Binsi, P.K.; Chakrabarti, R.; Triveni, A.; Joshy, C.G.; Lalitha, K.V.
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    GIS for Assessing Spatio-temporal Variations in Trawl Bycatch off Mangalore Coast
    (Society of Fisheries Technologists (India)Cochin, 2015) Dineshbabu, A.P; Thomas, Sujitha; Dinesh, A.C
    In India, trawl is the major gear contributing more than 50% of the marine fish landing and during 2008-2011, the average landing along the Indian coast was estimated at 1.7 million t. In India, fish brought from trawl fishery for human consumption in fresh or processed form is termed as commercial landing and the rest is called low valued bycatch (LVB). The increased demand for LVB from fish meal plants is an emerging threat for marine fish production in the future, since LVB was constituted mainly by juveniles of commercial fishes. The study showed that the percentage of LVB from the trawlers in India increased from 21% in 2008 to 23% in 2011. The results of the study identify some of the fishing grounds and seasons, in which the percentage of juvenile bycatch of the commercial species is very high. Implementing operational restrictions in the fishery in such areas will help in reviving the stock. The paper illustrates the utility of the spatiotemporal data in suggesting seasonal and spatial restrictions in fishing operations in tropical multispecies scenario.
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    Application of Monoclonal Antibody Based ELISA for Determining Circulating Specific Antibody in Rohu (Labeo rohita)
    (Society of Fisheries Technologists (India)Cochin, 2015) Babu, P. P. Suresh; Shankar, K. M; Honnananda, B. R.
    Since the MAb used in this study is specific for rohu immunoglobulin, similar ELISA can be developed for other pathogens to determine the pathogen specific antibody titre. More over the ELISA procedure is also compatible to determine the efficacy of vaccines in rohu for various pathogens. Further the sensitivity of the MAb can be enhanced by using the purified anti-rohu Ig MAb conjugated with the substrate as the primary antibody