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- ItemUtilization of fish bone as calcium supplement(Society of Fisheries Technologists (India), Cochin, 2003) Xavier, K.A.M.; Prabhu, U.A.; Nair, K.G.R.; Mathew, P.T.Fish bone is a good source of high quality calcium and phosphorus, which is being wasted now a days. Tuna canning industry generates substantial quantity of fish bone as waste. Fish bone collected from canning plant was deproteined using papain and fat content was removed either by KOH treatment or extraction using petroleum ether. It was dried and pulverised. The power thus obtained had calcium content of 43.2% and 38.2% respectively. The solubility of the calcium from these powders in HCL was very poor.