Peer Reviewed Journal Articles (National) (FP)
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Browsing Peer Reviewed Journal Articles (National) (FP) by Subject "Antioxidant activity"
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- ItemCharacterisation of hydrolysates prepared from engraved catfish (Nemapteryx caelata) roe by serial hydrolysis(Springer, 2016) Binsi, P.K.; Viji, P.; Panda, S.K.; Suseela Mathew; Zynudheen, A.A.; Ravishankar, C.N.Protein hydrolysates were prepared from defatted engraved catfish roe using alcalase enzyme by a two-stage serial hydrolysis process. The soluble hydrolysate formed after first stage of hydrolysis was removed (RH-1) and fresh enzyme was added at the same concentration to achieve further hydrolysis (RH-2). Further, compositional, surface-active and antioxidant properties of both hydrolysates were compared. The SDS-PAGE profile showed two distinct bands for RH-1, whereas no bands were visible for RH-2. On the other hand, gel filtration chromatography of the hydrolysates indicated 3–4 distinct fractions. Both the hydrolysates showed similar foam forming abilities, however, RH-1 exhibited poor foam stability. Emulsion properties of RH-1 were superior to that of RH-2. The major fractions eluted through gel filtration column were screened for antioxidant properties. Higher DPPH radical scavenging and metal chelating properties were observed for RH-1 second fragment, whereas FRAP and Fe2+ reducing power was highest for second fragment of RH-2.