Peer Reviewed Journal Articles (Inter.) (FP)
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- ItemResponse surface methodology model to optimize concentration of agar, alginate and carrageenan for the improved properties of biopolymer film(Springer, 2021-06-30)The present study intended to develop improved biopolymer film from seaweed polysaccharides. The quantity optimization of polysaccharides for the composite film was sought by empirical response surface methodology. To achieve the goal, Box– Behnken model was applied to the concentration of three independent variables, viz. agar (1.0–2.0% w/v), alginate (1.0–2.0% w/v) and carrageenan (1.0–2.0% (w/v). The glycerol was used as a plasticizer and kept constant (25% w/w) for total solid mass. The overall desirability function fits with the quadratic model at 99.78% level of significance for the optimization of agar (1.99% w/v), alginate (1.45 w/v) and carrageenan (2.0% w/v) to reach minimum water vapor permeability and maximum tensile strength, elongation at break and puncture resistance. The absolute residual error (1.04–3.37%) of experimental and predicted response was also validated. Attenuated total reflection-Fourier transform infrared spectroscopy confirmed the interactions such as stretching at 2900 cm− 1 region corresponded to C–H stretching vibration and an intensity peak observed at 1200 cm− 1 of AAC film corresponded to sulfate ester groups. The shift in crystalline nature of composite film was confirmed by XRD. The 3D image of atomic force microscopy showed layer-by-layer assembly of intermolecules at 310-nm resolution, and the characterized smooth surface has more functional application. The carrageenan and agar are found to be more responsible for the film properties such as moisture content, thickness, whiteness index, transparency, swelling and erosion than alginate.
- ItemDevelopment of biocompatible coating on Ti6Al4V implant materials using chitosan extracted from shellfish waste(Elsevier, 2021-06-14)The present study focuses on extracting chitosan from crustacean shells. The as-prepared chitosan was used to develop a uniform thin layer coating on titanium alloy (Ti6Al4V) implant material. Shellac, a natural resin was used intermediately for providing better adhesion. Taguchi optimization technique was used to optimize the control parameter values. Dipping duration and concentration of shellac were considered as control parameters and adhesion strength & thickness of the coating were considered as response characteristics. The concentration of shellac significantly affected the adhesion strength, whereas the thickness increased with an increase in dipping duration. Cytotoxicity tests and corrosion studies were performed on the samples coated with optimal values. The chitosan-coated sample was found as non-toxic and also showed better corrosion resistance.
- ItemCharacterization of Health Beverage Fortified with Peptides from Yellowfin Tuna(Taylor and Francis, 2021-09-15)Balanced nutrition is of utmost significance as it ensures health benefits in addition to basic nourishment. Present perilous pandemic outbreaks call for healthy and natural bioactive foods in our diet to strengthen the immune system. In the present study, bioactive peptides from yellowfin tuna red meat were used as supplement to formulate a health beverage. Malted grainbased health beverage formulated by RSM was added with tuna protein hydrolysate optimized for its bio-potency at 2.5, 5, 7.5 and 10% levels. Health beverage without peptide was kept as control. Sensorial studies recommended health beverage with 2.5% TPH to be acceptable. Characterization of the developed health formulation for its nutritional, functional, antioxidant, physical properties, in-vitro digestibility, as well as storage stability under ambient conditions (28°C) was carried out. Results suggest extensive scope of utilizing tuna cannery waste like red meat, for upgraded products to meet consumer specific nutritional demands that ensure health promoting effects.
- ItemAntioxidant properties of papain mediated protein hydrolysates from fresh water carps (Catla catla, Labeo rohita and Cirrhinus mrigala) and its application on inhibition of lipid oxidation in oil sardine mince during ice storage(Springer, 2021-03-12)Fish protein hydrolysates (FPH) from fresh water carps Catla catla, Labeo rohita and Cirrhinus mrigala were prepared with 5, 10, 15 and 20% degree of hydrolysis (DH) using papain enzyme. FPH were evaluated for antioxidant properties using in vitro assays such as DPPH free radical scavenging activity (at 10 mg/ml), ferric reducing antioxidant power assay (at 20 mg/ml) and linoleic acid peroxidation inhibition activity (at 10 mg/ml). Antioxidant properties of FPH varied with species and DH. The DPPH radical scavenging activity, linoleic acid peroxidation inhibition activity and ferric reducing antioxidant power (as absorbance at 700 nm) of FPH from carps was in the range of 59–92%, 52–85% and 0.388–0.663 respectively. Based on the overall antioxidant activity, FPH from C. catla with 20% DH was added to oil sardine mince at different concentration (0.1, 0.2 and 0.4%) and found to inhibit effectively the formation of peroxides and malonaldehyde in dose dependent manner. FPH from C. catla with 20% DH was fractionated using size exclusion chromatography and had three different peptide fractions with the approximate molecular weight of 6561–2106 Da (fraction 1), 1942–994 Da (fraction 2) and 935–383 Da (fraction 3). The present study showed promising results that the fish protein hydrolysates from fresh water carps muscle proteins can be used as natural antioxidants in food system. Production of fish protein hydrolysates with nutraceutical properties could be the way forward for better utilization and value addition.
- ItemCharacterization of chitin extracted from enzymatically deproteinized Acetes shell residue with varying degree of hydrolysis(Elsevier, 2021)Acetes shrimp is an unexploited tiny shrimp mainly landed as bycatch which is a good source for the recovery of protein and chitin. In the present study, the residual shell obtained after the hydrolysis of Acetes was used for the extraction of chitin by combining enzymatic and chemical treatments. Enzymatic hydrolysis with Alcalase was performed at different rates. Results showed that the protein removal efficiency increases with the increase in DH and the maximum deproteinzation was achieved at 30 % DH (93.68 %). The FTIR spectra showed two sharp bands for chemically prepared chitin and 30 % DH chitin at 1627–1629 and 1664 1665 cm 1 indicating that its alpha amorphous structure. The degree of N-acetylation was found to be higher in enzymatically prepared chitin in all different hydrolytic treatment rather than chemically prepared. The surface morphologies of chitin revealed the porous and nanofibrous structures for 30 % DH chitin and chemically prepared chitin.