Optimization of gelatin extraction from unicorn leatherjacket (Aleuterus monoceros) skin waste: response surface approach

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Date
2013
Authors
Mandakini Devi, H.
Sathish Kumar, K.
Kamei, G.
Jetender Kumar, J.
Mithilesh Kumar
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Abstract
Physical properties of gelatin extracted from Unicorn leatherjacket (Aluterus monoceros) skin, which is generated as a waste from fish processing industries, were optimised using Response Surface Methodology (RSM). A Box-Behnken design was used to study the combined effects of three independent variables, namely phosphoric acid (H3PO4) concentration (0.15–0.25 M), extraction temperature (40–50 °C) and extraction time (4–12 h) on different responses like yield, gel strength and melting point of gelatin. The optimum conditions derived by RSM for the yield (10.58%) were 0.2 M H3PO4 for 9.01 h of extraction time and hot water extraction of 45.83 °C. The maximum achieved gel strength and melting point was 138.54 g and 22.61 °C respectively. Extraction time was found to be most influencing variable and had a positive coefficient on yield and negative coefficient on gel strength and melting point. The results indicated that Unicorn leatherjacket skins can be a source of gelatin having mild gel strength and melting point.
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Keywords
Unicorn leatherjacket, Skin waste, Gelatin, Gel strength, Melting point, Response surface methodology
Citation
J. Food Sci. Technol. 52(2) : 976-983