A Simplified Scheme for Efficient Isolation and Identification of MRSA from Seafood and Aquatic Environment
A Simplified Scheme for Efficient Isolation and Identification of MRSA from Seafood and Aquatic Environment
Date
2020
Authors
Murugadas, V.
Joseph, Toms. C.
Lalitha, K. V.
Prasad, M. M.
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Fisheries Technologists (India)
Abstract
Methicillin resistant Staphylococcus aureus (MRSA) is
a versatile pathogen causing numerous clinical
illnesses and a potentially important organism of
food borne disease outbreaks. This pathogen has
increasingly been detected in several foods including
the seafood. However, protocol for efficient
isolation and identification in fisheries sector is not
developed, which is an environment at elevated salt
concentration. Therefore, experiments with 5 enrichment
broths and four selective agar plates in
different permutations and combinations were
tested for improved recovery of MRSA from seafood
of retail fish markets, fish landing centers and
aquaculture farms. This isolation and identification
scheme has been assessed for over 250 samples from
the fisheries sector and the results suggested that
this is the very simplified procedure for efficient
isolation and identification of MRSA and can be
accomplished in shortest period of time and as a
routine laboratory protocol. This may be adopted in
other food sectors after validation. The protocol
describes that samples enriched in tryptic soy broth
supplemented with 10% salt and 1% sodium
pyruvate followed by selective plating in BD ChromAgar MRSA II showed improved isolation of
MRSA compared to Oxacillin Resistance Screening
Agar Base ORSAB, Baird Parker Agar (BPA) + OX.
The protocol also describes the further simplified
steps of phenotypic and molecular confirmation of
MRSA in shortest period of time.
Description
Keywords
Sodium pyruvate, Trypticase soy broth, Aquatic Environment