Behaviour of marine microflora towards antibiotics - I. Sensitivity towards chlorotetracycline (CTC)

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Date
1971
Authors
Surendran, P. K.
MahadevaIyer, K.
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Publisher
Society of Fisheries Technologis(India)
Abstract
The sensitivity of 61 cultures of bacteria isolated from !fish towards chlortetracycline (CTC) at 5 ppm and 20 ppm levels lhas been determined on two solid media, viz; sea water agar (SW A) and a distilled water based medium consisting of peptone, beef extract, glucose and NaCI (PBGA). The cultures employed consisted of (i) gram-negative rods of marine origin viz; Achromobacler, Pseudomonas, Vibrio and Flavobacterium and (ii) gram positive orga• nisms viz; Micrococci and Corynebacterium. Depending on the inhibition zone diameter, the order of CTC sensitivity was found to be Pseudomonas < Vibrio < Achromobacter as determined on PBGA at CTC levels of 5 and 20 ppm. The SWA enhanced the development of resistance in majority of the cultures as compared to PBGA. PBG broth containing CTC at less than 10 ppm exerted a bacteriostatic effect only while that containing 10 ppm or more exerted a bactericidal effect on Achromobacter, Pseudomonas and Micrococci cultures. The significance of these results in fish preservation by CTC is discussed.
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Citation
Fish Technol.8(2):128-132