Peer Reviewed Journal Articles (National) (M,F&B)
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- ItemSeafood associated bacterial pathogens of public health significance: A brief review(Connect Journals, 2021)Seafood is perishable in nature and offers favourable medium for the growth of microorganism due to the presence of high content of water and nutrients. The absence of hygienic condition during production and processing results in proliferation of microorganisms thereby causing health risk to seafood handlers and consumers. The quality and safety of seafood is directly linked to the consumption of contaminated seafood. The potential hazards of microbial, chemical, or physical origin in farming, production, processing, or distribution stages, can compromise the quality of the product and becomes unacceptable for consumption. This review briefly describes the major seafood borne bacterial pathogens including emerging pathogens that can cause serious threat to food safety.
- ItemIdentification of Salmonella enterica subsp. enterica serovar Paratyphi B dT+ strains from seafood of Cochin retail markets(Society of Fisheries Technologists (India), 2021)
- ItemPrevalence, Antimicrobial resistance and Virulence profile of Salmonella from Aquaculture farms of central Kerala, India(Society of Fisheries Technologists (India), 2021)Salmonella belonging to Enterobacteriaceae is considered as the leading cause of food borne illness all over the world and products from aquaculture often act as vehicles of their transmission. In this study, a total of 150 samples including mud, water, fish/ shrimp and feed from 38 aquaculture farms from three districts of central Kerala viz., Thrissur, Ernakulam and Kottayam were screened for the presence of Salmonella. The overall prevalence of Salmonella in aquaculture farms was 7.9%. By serotyping, 100% of Salmonella isolates (n=8) from aquaculture were identified as Salmonella enterica subspecies enterica serovar Typhimurium with antigenic formulae 4,5,12: i: 1,2. None of the shrimp farms selected in this study harboured Salmonella. Antibiotic sensitivity testing revealed that 100% of S. Typhimurium were sensitive to the 17 antibiotics tested. Screening of the S. Typhimurium isolates for 11 virulence genes; invA, fimA, stn, spvC, sopB, mgtC, bcfC, csgD, avrA, hilA and phoP/phoQ belonging to Salmonella Pathogenicity Islands (SPI) 1 to 5 by PCR revealed that the isolates carried all the virulence genes except spvC gene, indicating its pathogenic potential. This study suggests that finfish farms are probable reservoirs of Salmonella with high virulence potential and can pose a potential threat to public health safety. Proper policies and regulations are to be adopted to control the occurrence of Salmonella in finfish culture systems.
- ItemSupercritical Fluid Extraction of Fish Oil – Recent Perspectives(International Journal of Current Microbiology and Applied Sciences (IJCMAS), 2020)Health benefits of omega-3 polyunsaturated fatty acids, such as Eicosapentaenoic acid (EPA) and Docosahexaenoic acid (DHA) has been well documented by researchers worldwide. Fish oil is reported to be one of the richest dietary sources of EPA and DHA. As a result, fish oil is being extracted on a wide scale globally using several methods. Recently, several innovative and sustainable techniques have been put forth by researchers for extraction of good quality fish oil. Supercritical fluid extraction is being recognised as one of the most feasible method for extracting high quality fish oil. The technique has been used extensively for extracting oil from fish meat as well as processing discards. The present review details on the extraction of fish oil using supercritical fluid extraction.
- ItemExpression Study of Pluripotency Marker Genes in Gold Fish, Carassius auratrus(International Journal of Current Microbiology and Applied Sciences (IJCMAS), 2020)Aquarium fishes, like medaka, zebrafish and goldfish offer an excellent model to conduct studies on molecular mechanisms of embryonic developmental pathways, patterns of genes involved in pluripotency and cell fate decision. To determine the possible candidate gene as an ideal pluripotency marker in goldfish, the comparative study on quantitative expression of a set of genes were conducted in the mid-blastula derived inner cell mass (ICM) and adult somatic cells. Present work explored the expression pattern of four standard pluripotency genes i.e. Nanog, Oct4, Sox and Pou2 in blastocytes (ICM) as well as somatic tissues of kidney, liver, heart, brain, muscle and mature gonads of goldfish using RT-qPCR. Absolute copy number of mRNA transcripts was calculated for each gene in every tissue. Significantly high (P<0.05) mRNA transcript number of all the genes was recorded in ICM, compared to other somatic tissues. Maternally inherited genes like Nanog, Pou2 and Oct4 were found to be highly expressive in ICM as well as in oocytes. The expression profile showed that Nanog andOct4 could belong to similar group where expression is limited to ICM and gonads whereas Pou2expressing in almost all the tissues, including ICM and adult gonads. Because of the low expression level in ICM than in the ovary, Sox2 exhibited expression in several somatic tissues apart from the gonad. Therefore these genes could be characterized as stem cell marker but not a pure pluripotency marker. The results showed that although all the genes compared in this study can be considered as stem cell markers but Nanog gene could be employed as a pure pluripotency marker for ES cell lines from goldfish.