Expression Study of Pluripotency Marker Genes in Gold Fish, Carassius auratrus
Expression Study of Pluripotency Marker Genes in Gold Fish, Carassius auratrus
Date
2020
Authors
Kumar, Abhay
Tripathi, Gayatri
Vimal, Bhartendu
Bedekar, Megha K.
Kumar, A. Pavan
Journal Title
Journal ISSN
Volume Title
Publisher
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
Abstract
Aquarium fishes, like medaka, zebrafish and goldfish offer an excellent model to conduct
studies on molecular mechanisms of embryonic developmental pathways, patterns of
genes involved in pluripotency and cell fate decision. To determine the possible candidate
gene as an ideal pluripotency marker in goldfish, the comparative study on quantitative
expression of a set of genes were conducted in the mid-blastula derived inner cell mass
(ICM) and adult somatic cells. Present work explored the expression pattern of four
standard pluripotency genes i.e. Nanog, Oct4, Sox and Pou2 in blastocytes (ICM) as well
as somatic tissues of kidney, liver, heart, brain, muscle and mature gonads of goldfish
using RT-qPCR. Absolute copy number of mRNA transcripts was calculated for each gene
in every tissue. Significantly high (P<0.05) mRNA transcript number of all the genes was
recorded in ICM, compared to other somatic tissues. Maternally inherited genes like
Nanog, Pou2 and Oct4 were found to be highly expressive in ICM as well as in oocytes.
The expression profile showed that Nanog andOct4 could belong to similar group where
expression is limited to ICM and gonads whereas Pou2expressing in almost all the tissues,
including ICM and adult gonads. Because of the low expression level in ICM than in the
ovary, Sox2 exhibited expression in several somatic tissues apart from the gonad.
Therefore these genes could be characterized as stem cell marker but not a pure
pluripotency marker. The results showed that although all the genes compared in this
study can be considered as stem cell markers but Nanog gene could be employed as a pure
pluripotency marker for ES cell lines from goldfish.
Description
Keywords
Pluripotency, ICM, Quantitative expression
Citation
Int.J.Curr.Microbiol.App.Sci (2020) 9(4): 639-649