Determination of electrophoretic subunit pattern and peptide mapping of collagen and collagen peptides extracted from skin of hammer head shark (Sphyrnae mokkaran)
Determination of electrophoretic subunit pattern and peptide mapping of collagen and collagen peptides extracted from skin of hammer head shark (Sphyrnae mokkaran)
| dc.contributor.author | Vijayan, D.K. | |
| dc.contributor.author | Raman, S.P. | |
| dc.contributor.author | Krishnamoorthy, E. | |
| dc.contributor.author | Mathew, S. | |
| dc.contributor.author | Ravishankar, C.N. | |
| dc.contributor.author | Anandan, R. | |
| dc.date.accessioned | 2019-08-28T06:39:04Z | |
| dc.date.available | 2019-08-28T06:39:04Z | |
| dc.date.issued | 2018 | |
| dc.description.abstract | SDS-PAGE is a simple moreover standard method for separation and identification of protein moiety based on their molecular size. In the present study, shark skin-acid soluble collagen (SSk-ASC) and shark skin-pepsin soluble collagen (SSk-PSC) were extracted from the skin of hammerhead shark. Subunit pattern of both extracted collagens were observed using SDS-PAGE. From the electrophoretic patterns both the extracts were identified as they belong to type I. Collagen peptides were prepared from SSk-ASC by enzymatic digestion, followed by subsequent fractionation using gel filtration chromatography and anion exchange chromatography. The relationship between molecular weight distributions along with the antioxidant activity of the fractions were measured using SDS-PAGE analysis and ABTS radical scavenging assay. The results of evaluation revealed that the fractions with smallest fragments exhibiting maximum antioxidant activity. | en_US |
| dc.identifier.citation | SciFed J. Analyt. Biochem. 1(3):1-8 | en_US |
| dc.identifier.uri | http://drs.cift.res.in/handle/123456789/4240 | |
| dc.language.iso | en | en_US |
| dc.title | Determination of electrophoretic subunit pattern and peptide mapping of collagen and collagen peptides extracted from skin of hammer head shark (Sphyrnae mokkaran) | en_US |
| dc.type | Article | en_US |
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