Purification and characterisation of phosphorylase from muscle of Tilapia(Tipapia mosambica)
Purification and characterisation of phosphorylase from muscle of Tilapia(Tipapia mosambica)
Date
1977
Authors
Mukundan, M.K..
Nair, M.R.
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Fisheries Technologis(India)
Abstract
Phosphorylase from muscle of tilapia (Tilapia mosambica)
was extracted by water and purified by ammonium sulphate
precipitation, centrifugation and repeated recrystallisation. Electrephorogram
of the enzyme preparation showed a single band
near origin. The enzyme showed optimum pH and temperature
at 6.1 and 37°C respectively. Glucose and glucose-6-phosphate
were found to be competitive inhibitors of the enzyme. Maltose
and starch acted as primers for the phosphorylase reaction
like glycogen.02
Description
Keywords
Citation
Fish Technol.14(1):1-6