Isolation and molecular characterisation of atypical enterotoxigenic bacillus cereus with negative voges-proskauer reaction from Indian white shrimp fenneropenaeus indicus (H. Milne Edwards, 1837)
Isolation and molecular characterisation of atypical enterotoxigenic bacillus cereus with negative voges-proskauer reaction from Indian white shrimp fenneropenaeus indicus (H. Milne Edwards, 1837)
Date
2013
Authors
Das, S.
Lalitha, K.V.
Thampuran, N.
Journal Title
Journal ISSN
Volume Title
Publisher
ICAR
Abstract
Enterotoxigenic Bacillus cereus is one of the important pathogenic organisms, which causes two distinct type of food
poisoning in human beings viz., emetic type and diarrhoeal type. As per biochemical tests, B. cereus is generally
Voges-Proskauer (VP) positive. In the present study, two enterotoxigenic B. cereus isolates from white shrimp
(Fenneropenaeus indicus) with negative VP reaction have been reported. Both the isolates were confirmed as B. cereus by
other biochemical tests as well as molecular methods like PCR and 16S rRNA gene sequencing. They were also found to
produce diarrhoeal enterotoxin by reverse passive latex agglutination (RPLA) test. The enterotoxin producing genes, hbla
and entb were detected in both the isolates
Description
Keywords
bacillus cereus, enterotoxin, voges-proskauer(VP) negative
Citation
Indian Journal of Fisheries 2013: 60(4), 113-117