In vitro anti-cervical cancer effect of proteoglycans isolated from deep sea shark cartilage by inducing apoptosis and cell cycle arrest
In vitro anti-cervical cancer effect of proteoglycans isolated from deep sea shark cartilage by inducing apoptosis and cell cycle arrest
Date
2017
Authors
Ajeeshkumar, K.K.
Vishnu, K.V.
Asha, K.K.
Remya Kumari, K.R.
Reshma, J.
Suseela Mathew
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The present study was aimed at studying in vitro anti-cervical cancer effect of proteoglycans
(PGs) isolated from deep sea shark. Anti-proliferative activity and apoptotic involvement were
confirmed by following assays viz, the cell viability assay by MTT assay, morphological analysis
and colony formation assay. The sample showed a significant cytotoxic activity for HeLa cells
and results showed that the PGs at a concentration of 250μg/mL inhibit the colony formation
capacity. Apoptosis induction was confirmed by the chromatin condensation assay by Hoechst
33342 nuclear staining. A number of apoptotic HeLa cells displayed a round and shrunken cell
body and chromatin condensation inside the nucleus suggesting that PGs-induced apoptosis
caused cell death. Apoptosis of HeLa cells was further verified by annexin V-FITC (fluorescein
isothiocyanate) and PI (propidium iodide) staining by flow cytometry. PGs (250 μg/ml) were
found to induce apoptosis and the ratio of apoptotic cells were 30.5%. Cell cycle arrest at G1/M
and G2/M phase confirmed the cell death due to cell cycle arrest. Our study determined that
proteoglycans effectively induced apoptosis and caused cell cycle arrestestablishing their anti-proliferative
effect against cervical cancer.
Description
Keywords
Proteoglycans, Shark cartilage, Apoptosis, Cell cycle arrest
Citation
International Journal of Development Research Vol. 07(12): 17472-17478