Studies on isolation of Salmonella from seafoods - I. Comparison of enrishment and selective media for recovery of salmonella from fish

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Date
1972
Authors
Arul James, M.
MahadevaIyer, K.
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Publisher
Society of Fisheries Technologis(India)
Abstract
Three enrichment broths and six plating media were compared for efficiency of detection of Salmonella in the presence of high numbers of Conforms (105/ml) and proteus (103/m1) from artificially inoculated fish samples. Recovery experiments with Salmonellaanatum S. typhimurium and S. enteritidis indicated that the two enrichment broths viz. Dulcitol Selinite (DSE) and Selinite Cystine (SC) were equally efficient. Further, the viability of Salmonella, inoculated into fish muscle and kept at 40 C for 48 hours, was found to be not affected by the low temperature storage. Selective plating media like Xylose Lysine Deoxycholate agar (XLD), Brilliant Green Sulphadiazine agar (BGS) and Brilliant Green agar (BG) were found to be superior in performance to Salmonella-Shigella agar: (SS) and Bismuth Sul phite agar (BiS).
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Fish Technol.9(2):115-121