Detection of listeria monocytogenes from freshwater fish, prawn and chicken meat by direct nested PCR
Detection of listeria monocytogenes from freshwater fish, prawn and chicken meat by direct nested PCR
Date
2011
Authors
Das, S.
Singh, V.P.
Ltu, K.
Kathiresan, S.
Sharma, B.
Bhilegaonkar, K.N.
Journal Title
Journal ISSN
Volume Title
Publisher
Asian Fisheries Society
Abstract
Three different methods of processing samples from freshwater fish, prawn and chicken meat were compared with nested PCR for the detection of Listeria monocytogenes. Nested PCR failed to detect the organism from freshwater fish, prawn and chicken meat when processing was done by boiling lysis method, but it could detect the organisms from freshwater fish, prawn and chicken meat up to the spiking level of 104, 103 and 105 cfu.g-1, respectively while processing of sample was done by phenol extraction without enrichment. However, using phenol extraction after enrichment, the sensitivity was found higher than without enrichment and it was possible to detect up to the spiking level of 102, 10 and 103 cfu.g-1 in case of fish, prawn and chicken meat, respectively.
Description
Keywords
Listeria monocytogenes, freshwater fish, direct nested PCR
Citation
Asian Fisheries Science 2011: 24(2), 432-442