Studies on the electrophoretic patterns of fish muscle myogens
Studies on the electrophoretic patterns of fish muscle myogens
| dc.contributor.author | Devadasan, K. | |
| dc.contributor.author | Nair, M.R. | |
| dc.date.accessioned | 2013-06-18T08:28:31Z | |
| dc.date.available | 2013-06-18T08:28:31Z | |
| dc.date.issued | 1971 | |
| dc.description.abstract | Observations made on the disc electrophoretic patterns of muscle myogens of a variety of Indian fish and shellfish are reported and their usefullness in species identification is discussed. Species studied were prawns (Metapenaeous dobsoni), oil sardine (Sardinella longiceps), mackerel (Rastrelliger kanagurta), lactarius (Lactarius lactarius), mullet (Mugil dussumieri), barbus (Barbus carnaticus) and tilapia (Tilapia mosambica). Muscle was extracted with chilled distilled water (1:1 w/v) in a Waring blender for 2 min, the homogenate centriguged at 5000 rev/min for 30 min at 0 degrees C, and the supernatant electrophoresed in 7.5% gel by the method of Ornstein and Davies ['Disc electrophoresis' (1964) Distillation Products Industries, Rochester, New York, USA] using bromophenol blue as marker dye. Electrophoresis was completed in 40 min (with 6.6 mA/gel tube at 150-200 V). Gels were then removed, stained in 0.1% solution of Amido black IOB in 7% acetic acid for 30 min. Excess dye was removed by washing in 7% acetic acid; developed gels were kept in 7% acetic acid and photographed. Results indicated that a characteristic band pattern was produced which was species specific and was not altered by storage of fish in ice. So disc electrophoresis can be reliably used in fish taxonomy | en_US |
| dc.identifier.citation | Fishery Technology 1971:8(1), 80-82 | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/237 | |
| dc.language.iso | en | en_US |
| dc.publisher | Society of Fisheries Technologists (Indai) | en_US |
| dc.subject | fish | en_US |
| dc.subject | meat | en_US |
| dc.subject | shellfish | en_US |
| dc.subject | science | en_US |
| dc.subject | taxonomy | en_US |
| dc.subject | taxonomy | en_US |
| dc.title | Studies on the electrophoretic patterns of fish muscle myogens | en_US |
| dc.type | Article | en_US |
Files
Original bundle
1 - 1 of 1
- Name:
- STUDIES ON THE ELECTROPHORETIC PATTERNS OF FISH MUSCLE MYOGENS.pdf
- Size:
- 221.44 KB
- Format:
- Adobe Portable Document Format
- Description:
License bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- license.txt
- Size:
- 201 B
- Format:
- Item-specific license agreed upon to submission
- Description: