Peer Reviewed Journal Articles (National) (B&N)
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- ItemVariations in lipid profile of of the marine microalga Nannochloropsis salina in four different culture media(Indian Council of Agricultural Research, 2022-09-30)The present work envisages close monitoring of variations in lipid content and fatty acid profile of the marine microalga Nannochloropsis salina, by growing it in 4 different culture media. Pure cultures of microalga were collected from the marine hatchery complex of the ICAR-Central Marine Fisheries Research Institute, Kochi and cultured in four different media viz., f/2 medium (Guillard, 1975), Miquel’s medium (Miquel, 1892), Walne’s medium (Walne, 1974) and Chu#10 medium (Chu et al., 1942). Standard algal culture conditions viz, salinity 33 ppt, pH 8.0, culture room temperature of 25ºC, light intensity of 1500 lux with a photoperiod of 12 h and Light:Dark cycle of 12 h were maintained. Lipids extracted were subjected to gas chromatography to ascertain their fatty acid profile. Both Miquel’s and f/2 media produced the maximum amount of lipid content (40.2 and 40% respectively). Total saturated fatty acids ranged from 39.17% (Miquel’s medium) to 84.45% (Chu#10). For monounsaturated fatty acids, the highest value was obtained for Miquel’s medium (42.79%) and minimum for Chu#10 (10.55%). Polyunsaturated fatty acids accounted for over 23.50% of the total fatty acids in Walne’s medium followed by Miquel’s (18.04%), f/2(12.76%) and Chu#10(5.00%) media. One way analysis of variance of the data brought to light that the medium composition can influence the fatty acid pattern of microalgae significantly.
- ItemThe role of carotenoids in enhancing the health of aquatic organisms(International Journal of Fisheries and Aquatic Studies, 2021)Carotenoids constitute a class of pigments derived from photosynthetic plants, algae, bacteria and some fungi. Carotenoids act as potent antioxidants scavenging reactive oxygen species. They hold a vital role in the antioxidant defense system of humans. Several studies have shown carotenoid can increase the growth overall well being of aquatic animals. This review examined the effect of carotenoid in aquatic organisms on growth, survival, immunity, pigmentation and reproductive performance. The review recommends the use of carotenoid as feed additive for enhance the overall improvement of the aquatic organisms.
- ItemFucoxanthin Content and Antioxidant Activity in Supercritical CO2, Enzymatic and Natural Hydrophobic deep Eutectic Solvent Extracts of Sargassum wightii Seaweed(Society of Fisheries Technologists (India), 2021)Brown seaweeds are potential sources of bioactive compounds which can be beneficial in the development of novel nutraceuticals and cosmeceuticals. Green chemistry techniques, namely supercritical fluid (SFE), enzymatic and natural deep eutectic solvent (NaDES) extraction, were employed on Sargassum wightii seaweed. LC-MS/MS estimation revealed that the SFE technique could extract the maximum amount of fucoxanthin (3.00±0.04 mg g-1 seaweed), followed by NaDES (1.7±0.006 mg g-1 seaweed) and enzymatic extraction (0.17±0.003 mg g-1 seaweed). However, total phenolic content, expressed as mg gallic acid equivalent g-1 extract, was highest in enzymatic (90±2.6), followed by SFE (61.02 ±1.4) and NaHDES (55.35± 2.06) extracts. The DPPH free radical scavenging activity was highest in the SFE extract (IC50 2.07±0.04 μg gallic acid equivalent). There was no significant difference in the DPPH free radical scavenging activity between the enzymatic (IC50 13±3.24 μg gallic acid equivalent) and NaDES extracts (IC50 16.64±2.04 μg gallic acid equivalent). Interestingly, ABTS free radical scavenging activity was highest in the enzymatic extract (IC50 11±2.06 μg Trolox equivalent), followed by SFE and NaDES extracts. The study reveals that all the three methods are excellent alternatives to harmful organic solvents. The result indicates that SFE is the best method among all the employed techniques.
- ItemANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES OF LIVER OILS OF FOUR SHARK SPECIES FROM INDIAN EEZ(WILEY, 2008)The analgesic and anti-inflammatory properties of liver oils of four different sharks, namely Neohariotta raleighana, Centrosymnus crepidater, Apristurus indicus and Centrophorus scalpratus, captured from the Arabian Sea and the Indian Ocean were evaluated. While the analgesic property was determined using the acetic acid-induced mouse writhings and hot-plate reaction time, the anti-inflammatory activity was evaluated using the formalin-induced rat-paw edema. The oils examined were found to possess significant (P < 0.05) analgesic activity against acetic acid-induced writhings and hot-plate reaction in mice. In the formalin-induced edema, a significant (P < 0.05) inhibition of inflammation was observed between the 2nd and 4th hour showing 58–65% inhibition. These results suggest that liver oils of sharks from Indian waters are effective as analgesic and anti-inflammatory agents. The role of lipid components (squalene, alkylglycerols and polyunsaturated fatty acids) on anti-inflammatory and antinociceptive properties is highlighted. Inhibition of the synthesis of prostaglandins and other inflammatory mediators which probably account for the properties is discussed.
- ItemPROXIMATE COMPOSITION, AMINO ACID AND FATTY ACID PROFILES OF COMMERSON’S ANCHOVY (STOLEPHORUS COMMERSONII) AND INDIAN MACKEREL (RASTRELLIGER KANAGURTA)(connect Journals, 2012)Proximate composition, amino acid profile, fatty acid composition and mineral status of Commerson’s anchovy (Stolephorus commersonii) and Indian Mackerel (Rastrelliger kanagurta) were examined. It is observed that the amino acid composition and fatty acid profile of low value Commerson’s anchovy (Stolephorus commersonii) is comparable to that of Indian Mackerel (Rastrelliger kanagurta). It is found to be rich in all essential amino acids and polyunsaturated fatty acids in balanced proportions. It is also containing minerals, macro elements and trace elements in significant proportions. The results of the present study indicate that both Commerson’s anchovy and Indian Mackerel contain all nutrients in balanced proportions essentially required for the growing children and pregnant women.