Cryopreservation of Brown Trout (Saltno trutta fario) Sperm: The Influence of Extender Composition and Fertilization Procedure
Cryopreservation of Brown Trout (Saltno trutta fario) Sperm: The Influence of Extender Composition and Fertilization Procedure
Date
1999
Authors
Gopalakrishnan, A
Thakur, K.L
Ponniah, A.G
Kumar, K
Dayal, R
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Fisheries Technologists (India)Cochin
Abstract
Seven extenders were tested to check their relative efficiency in cryopreserving the
milt of brown trout (Saltno trutta fario). Hatching rates equal to that of control (p > 0.05)
were obtained when a cryodiluent containing egg yolk (2%) and dimethylsulphoxide (10%)
was used. Removal of coelomic fluid prior to the addition of thawed milt and addition
of 5 mM theophylline to the "Dexter" solution enhanced the hatching percentage. The sperm
density varied from 9.83 to 18.41 x 109 sperms/ml.
Description
Keywords
Cryopreservation, brown trout, activator, cryo diluent, extender, milt
Citation
Fishery Tech 36(2):104-109