Prevalence and characterization of typical and atypical escherichia coli from fish sold at retail in Cochin, India

Simple item page
dc.contributor.authorThampuran, N.
dc.contributor.authorSurendraraj, A.
dc.contributor.authorSurendran, P.K.
dc.date.accessioned2014-02-11T10:03:38Z
dc.date.available2014-02-11T10:03:38Z
dc.date.issued2005
dc.description.abstractEscherichia coli is a common contaminant of seafood in the tropics and is often encountered in high numbers. The count of E. coli as well as verotoxigenic E. coli O157:H7 was estimated in 414 finfish samples composed of 23 species of fresh fish from retail markets and frozen fish from cold storage outlets in and around Cochin, India. A total of 484 presumptive E. coli were isolated, and their indole-methyl red-Voges-Proskauer-citrate (IMViC) pattern was determined. These strains were also tested for labile toxin production by a reverse passive latex agglutination method and checked for E. coli serotype O157 by latex agglutination with O157-specific antisera. Certain biochemical marker tests, such as methylumbelliferyl- beta -glucuronide (MUG), sorbitol fermentation, decarboxylase reactions, and hemolysis, which are useful for screening pathogenic E. coli, were also carried out. Results showed that 81.4% of the E. coli isolates were sorbitol positive. Among this group, 82% were MUG positive, and 14.46% of the total E. coli isolates showed human blood hemolysis. None of the isolates were positive for agglutination with E. coli O157 antisera nor did any produce heat-labile enterotoxin. This study indicates that typical E. coli O157 or labile toxin-producing E. coli is absent in the fish and fishery environments of Cochin (India). However, the presence of MUG and sorbitol-negative strains that are also hemolytic indicates the existence of aberrant strains, which require further investigation.en_US
dc.identifier.citationJournal of Food Protection 2005: 68(10), 2208-2211en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1422
dc.language.isoenen_US
dc.publisherInternational Association for Food Protectionen_US
dc.subjectEscherichia colien_US
dc.subjectfish sold at retailen_US
dc.subjecttypical and atypicalen_US
dc.titlePrevalence and characterization of typical and atypical escherichia coli from fish sold at retail in Cochin, Indiaen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Prevalence and Characterization of Typical and Atypical Escherichia coli from Fish Sold at Retail in Cochin, India.pdf
Size:
2.66 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Journal articles (MFB)