Detection of vibrio cholerae by polymerase chain reacrion method
Detection of vibrio cholerae by polymerase chain reacrion method
Date
2003
Authors
Thampuran, N.
Surendran, P.K.
Journal Title
Journal ISSN
Volume Title
Publisher
Society of Fisheries Technologists(India),Cochin
Abstract
The food safety and quality control in the seafood processing industry necessitates rapid detection of pathogens. Most of the pathogens occur in the food in small numbers only so that detection becomes very difficult. Some of the pathogens like Vibrio cholerae also exist in the so called "viable but non-culturable" state in the environment so that ordinary biochemical protocol becomes unsuccessful to detect their presence. Molecular methods such as polymerase chain reaction (PCR) has the advantage over the existing biochemical detection method in detection very small numbers of stressed cells of these pathogens and that too in a relatively short time. A PCR method is described to detect Vibrio cholerae in fish by amplifying a 564 bp fragment of the CTX gene.
Description
Keywords
Polymerase chain reaction, seafood safety, vibrio cholerae
Citation
Proceedings of the Symposium on Seafood Safety - Status and Strategies, 28-30 May 2002, Cochin, India ed. by Surendran, P.K. et. al, 550-555