Cloning and heterologous expression of ectoine biosynthesis genes from bacillus halodurans in escherichia coli

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dc.contributor.authorRajan, L.A.
dc.contributor.authorJoseph, T.C.
dc.contributor.authorThampuran, N.
dc.contributor.authorJames, R.
dc.contributor.authorKumar, K.A.
dc.contributor.authorViswanathan, C.
dc.contributor.authorBansal, K.C.
dc.date.accessioned2013-12-20T07:01:19Z
dc.date.available2013-12-20T07:01:19Z
dc.date.issued2008
dc.description.abstractThe genes involved in the biosynthetic pathway of ectoine (2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid) from Bacillus halodurans were cloned as an operon and expressed in E. coli. Analysis of the deduced ectoine biosynthesis cluster amino acid sequence revealed that the ectoine operon contain 2,389bp, encoded by three genes; ectA, ectB and ectC that encode proteins of 189, 427 and 129 amino acids with deduced molecular masses of 21,048, 47,120 and 14,797Da respectively. Extracts of induced cells showed two bands at 41kDa and 17kDa, possibly corresponding to the products of the later two genes. However the expression of ectA gene could not be ascertained by SDS-PAGE. The activity of the ectA protein was confirmed by an acylation assay. The transgenic E. coli accumulated upto 4.6mg ectoine/l culture. This is the first report of an engineered E. coli strain carrying the ectoine genes of the alkaliphilic bacterium, B. halodurans.en_US
dc.identifier.citationBiotechnology Letters 2008: 30(8) 1403-1407en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1027
dc.language.isoenen_US
dc.publisherSpringer Science+Business Mediaen_US
dc.subjectBacillus haloduransen_US
dc.subjectcompatible solutesen_US
dc.subjectectoine genesen_US
dc.subjectosmoregulationen_US
dc.titleCloning and heterologous expression of ectoine biosynthesis genes from bacillus halodurans in escherichia colien_US
dc.typeArticleen_US
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