A study on the intestinal lipase of Indian major carp Labeo rohita

Simple item page
dc.contributor.authorNayak, J.
dc.contributor.authorNair, P.G.V.
dc.contributor.authorMathew, S.
dc.contributor.authorAmmu, K.
dc.date.accessioned2014-08-07T06:10:38Z
dc.date.available2014-08-07T06:10:38Z
dc.date.issued2004
dc.description.abstractLipase from rohu(Labeo rohita, Cyprinidae) intestine was extracted, fractionated with ammonium sulphate and purified on Sephadex G100 column. It was found that the specific activity increased 3.6 times after ammonium sulphate precipitation and Sephadex G-100 Chromatography of crude extract. Lipase activity at different levels of ammonium sulphate showed lower activity. Fractions precipitated with 80% saturated ammonium sulphate has the maximum activity and no activity was detected in the filtrate from this fraction. Lipase was found to have optimum activity at temperature 45 degree C and at pH 7.0. The amino acid composition of the enzyme showed that lipase had high amount of polar amino acids than non polar amino acid content.en_US
dc.identifier.citationAsian Fisheries Science 2004:17,333-340en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1815
dc.language.isoenen_US
dc.publisherAsian Fisheries Society, Manila, Phillipinesen_US
dc.subjectlipolytic enzymesen_US
dc.subjectLabeo rohitaen_US
dc.titleA study on the intestinal lipase of Indian major carp Labeo rohitaen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
a study on the intestinal lipase.pdf
Size:
542.19 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Peer Reviewed Journal Articles (Inter.) (B&N)